Stemgent® Dox Inducible Reprogramming Lentivirus Mouse Oct4 (conc)
The Stemgent® Dox Inducible Reprogramming Lentivirus Mouse Oct4 (conc) provides the mouse transcription factor mOct4 for use with Sox2, c-Myc and Klf4 to reprogram somatic cells into an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell1.
1. Brambrink et. al., Cell Stem Cell 2: 151-159 (2008).
Stemgent® is partnering with Sigma® Life Science to distribute our proven collection of reprogramming lentiviruses for induced pluripotent stem cell (iPSC) generation. With Sigma’s global distribution network, you can easily access Stemgent lentivirus products regardless of your location. And you can continue to rely on the experienced reprogramming scientists at Stemgent to provide technical expertise from experimental design through data interpretation, and each step in between.
Ordering Stemgent® Lentivirus Reprogramming Products
How to place your order
Orders can be placed either through the Sigma or Stemgent, via phone, fax, or website.
Stemgent ordering channels
- Website: www.stemgent.com
- Phone (US): 1-877-228-9783
- If you order through Stemgent, a Sigma representative will contact you to confirm your order.
Sigma ordering channels
- Website: www.sigmaaldrich.com
- Phone (US): 1-800-325-3010; for orders outside the US, contact your local Sigma customer service center
- Fax (US): 1-800-325-5052; for orders outside the US, contact your local Sigma customer service center
How Lentivirus Products will Arrive
Whether ordered from Sigma or Stemgent, all Lentivirus orders will be supplied by Sigma. Your order will arrive on dry ice in a Sigma shipping box.
Billing
You will receive an invoice from Sigma for any Reprogramming Lentiviruses that you order. The invoice can be paid just as any other Sigma invoices are paid by your company or institution.
Stemgent® Dox Inducible Reprogramming Lentivirus Mouse Oct4 (conc)
Stemgent® Dox Inducible Reprogramming Lentivirus Mouse Oct4 is provided as prepackaged lentivirus particles for inducible reprogramming of mouse fibroblast cells. This VSV-G pseudotyped lentivirus is capable of infecting both dividing and non-dividing cells. Under the control of the doxycycline (Dox)-inducible tetO operator and in the presence of the reverse tetracycline transcriptional activator (rtTA) and doxycycline, induced expression of mSox2, mOct4, mc-Myc, and mKlf4 has been shown to direct a variety of differentiated cells into induced pluripotent stem (iPS) cells with embryonic stem (ES) cell-like properties. Proper isolation and subsequent injection of these iPS cells into blastocysts can generate mice that carry all four inducible viruses, which can be reactivated upon the addition of Dox.
References
- Brambrink, T., Foreman, R., Welstead, G.G., Lengner, C.J., Wernig, M., Suh, H., and Jaenisch, R. (2008). Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2, 151-159.
For research use only. Not for use in diagnostic procedures. Not for resale.
Stemgent® Dox Inducible Reprogramming Lentivirus Mouse Oct4 (conc)
| Size / Quantity | ST070003-100UL |
| Sterility | Tested to be free of bacteria. TSB (tryptic soy broth) was inoculated with each virus and grown for 4 days at 30°C and 3 days at room temperature to assay for bacterial growth. |
| Storage and Stability | Store at -70°C for up to 9 months. Keep frozen until use. Avoid freeze thaw cycles. |
| Quality Control | Viral concentration determined by p24 (capsid antigen) ELISA assay. Transcription factor protein expression verified by immunocytochemistry analysis of transduced mouse embryonic fibroblasts (MEFs). |
| Safety Precautions | The greatest safety risk associated with viral delivery systems stems from the potential generation of recombinant viruses that are capable of autonomous replication during the packaging process. The Stemgent® Reprogramming Lentivirus platform eliminates these hazards by combining a disabled viral genome with a unique manufacturing process. Also, gene functions that facilitate the enclosing of the silencing sequence in a viral capsid (e.g., Gag, Pol, Env) are distributed into multiple helper plasmids (which do not contain significant regions of homology) during packaging. This tactic further minimizes the probability of recombination events that might otherwise generate viruses capable of autonomous replication. With these safety measures in place, the Stemgent® Reprogramming Lentivirus particles can be employed in standard Biosafety Level 2 tissue culture facilities and should be treated with the same level of caution as with any other potentially infectious agent. Also, please note that the c-Myc and Klf4 proteins are reported to demonstrate oncogenic properties. |
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| Restricted Use | For restrictions related to the use of this product please refer to the accompanying Restricted Use License. |
| Notice to Purchaser | Purchaser represents and warrants that it will use Stemgent® Dox Inducible Reprogramming Lentivirus Mouse Oct4 (conc) purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
| Sigma Product Statement | Stemgent® Reprogramming Lentivirus products are sold through Sigma-Aldrich Inc. Sigma-Aldrich Inc. warrants that its products conform to the information contained in this and in other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see the reverse side of the invoice or packing slip. |
Have you published research involving the use of our products? If so, we'd love to hear about it. Please let us know!
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