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Stemgent offers advanced stem cell-qualified reagents and
tools developed by some of the world's leading stem cell scientists.
Stemolecule™ BIX01294
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Product Overview
BIX01294 is a selective inhibitor of G9a histone methyl transferase (G9a HMTase) that impairs G9a HMTase and generation of H3K9me2 in vitro1. G9a HMTase regulates gene expression including one of the pluripotency genes Oct42. Shi and colleagues (2008) found that BIX01294 enhances reprogramming efficiency of neural progenitor cells to the same levels as when four transcription factors (Oct4, Klf4, Sox2 and c-Myc) were introduced to somatic cells for generation of induced pluripotent stem cells2.
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References
- Kubicek, S. et al., Mol. Cell. 3rd ed., 25:473-481 (2007)
- 2. Shi, Y. et al., Cell Stem Cell, 2:525-528 (2008).
For research use only. Not for use in diagnostic procedures. Not for resale.
Product Specifications
| Chemical Formula |
C28H38N6O2
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| Structure |
![[structure graphics]](http://commerce.stemgent.com/products/04-0002/images/structure.png)
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| Molecular Weight |
490.64
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| CAS Number |
935693-62-2
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| Purity |
>94.9% by HPLC analysis
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| Formulation |
Pale yellow solid
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| Solubility |
Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 1019 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
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| Storage |
4°C protected from light. Following reconstitution, store aliquots at -20°C
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| Stability |
Stock solutions stable at -20°C for 6 months
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| Quality Control |
The purity of BIX01294 was determined by HPLC analysis. The accurate mass and structure of BIX01294 was determined by mass spectrometry and NMR, respectively. Cellular toxicity of BIX01294 was tested by using HeLa and HEK293 cells.
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Technical Documents
Specifications
MSDS
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