Stemolecule™ BIX01294

Stemolecule™ BIX01294

BIX01294 is a selective inhibitor of G9a histone methyl transferase (G9a HMTase) that impairs G9a HMTase and generation of H3K9me2 in vitro¹. G9a HMTase regulates gene expression including one of the pluripotency genes Oct4². Shi and colleagues (2008) found that BIX01294 enhances reprogramming efficiency of neural progenitor cells to the same levels as when four transcription factors (Oct4, Klf4, Sox2 and c-Myc) were introduced to somatic cells for generation of induced pluripotent stem cells².

View Poster

References

1. Kubicek, S. et al., Mol. Cell. 3rd ed., 25:473-481 (2007)
2. 2. Shi, Y. et al., Cell Stem Cell, 2:525-528 (2008).

Related Products
Stemolecule™ PD0325901 (Cat. No. 04-0006)
Stemolecule™ R(+)Bay K 8644 (Cat. No. 04-0013)

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ BIX01294

Chemical Formula	C28H38N6O2
Structure
Molecular Weight	490.64
CAS Number	935693-62-2
Purity	>94.9% by HPLC analysis
Formulation	Pale yellow solid
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 1019 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	4°C protected from light. Following reconstitution, store aliquots at -20°C
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of BIX01294 was determined by HPLC analysis. The accurate mass and structure of BIX01294 was determined by mass spectrometry and NMR, respectively. Cellular toxicity of BIX01294 was tested by using HeLa and HEK293 cells.
References	Kubicek, S. et al., Mol. Cell. 3rd ed., 25:473-481 (2007) 2. Shi, Y. et al., Cell Stem Cell, 2:525-528 (2008).

Download PDF

MSDS

• Material Safety Data Sheet

Wiki

• Small Molecules
• Stemolecule™ BIX01294