Stemolecule™ CHIR99021

Stemolecule™ CHIR99021

The aminopyrimidine CHIR99021 is the most selective inhibitor of glycogen synthase kinase 3β (GSK3β) reported to date^{1, 2}. Unlike alsterpaullone, kenpaullone, SB214763, and SB415286 that are potent inhibitors of GSK-3, CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) with 350-fold selectivity toward GSK-3β compared to CDKs with a K_i of <10 nM in vitro³.

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References

1. Ring D. B. et al., Diabetes, 52:588-595 (2003).
2. Hall R. K. et al., J Biol Chem, 275:30169-30175 (2000).
3. Finlay D. et al., BMC Molecular Biology, 5:15.

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For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ CHIR99021

Chemical Formula	C22H18Cl2N8
Structure
Molecular Weight	465.34
Purity	>95% by HPLC analysis
Formulation	Off-white solid
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 430 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	4°C protected from light. Following reconstitution, store aliquots at -20°C
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of CHIR99021 was determined by HPLC analysis. The accurate mass and structure of CHIR99021was determined by mass spectrometry and NMR, respectively. Cellular toxicity of CHIR99021 was tested by using HeLa and HEK293 cells.
References	Ring D. B. et al., Diabetes, 52:588-595 (2003). Hall R. K. et al., J Biol Chem, 275:30169-30175 (2000). Finlay D. et al., BMC Molecular Biology, 5:15.

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