Spec Sheet

Stemolecule™ Sodium Butyrate

Catalog #	Size	Price (USD)	Quantity
04-0005	500 mg	$49.00	Qty:

In cultured mouse and human cells, sodium butyrate has been shown to inhibit both the mRNA and protein content of cyclin D1.¹ Sodium butyrate is a known inhibitor of histone deacetylases.^2,3

1. Lallemand, F., Courilleau, D., Sabbah, M., Redeuilh, G., Mester, J. (1996) Direct inhibition of the expression of cyclin D1 gene by sodium butyrate. Biochem Biophys Res Commun 229: 163-9.
2. Kruh, J. (1982) Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol Cell Biochem 42: 65-82.
3. Ware C.B., Wang L., Mecham B.H., Shen L., Nelson A.M., Bar M., Lamba D.A., Dauphin D.S., Buckingham B., Askari B., Lim R., Tewari M., Gartler S.M., Issa J.P., Pavlidis P., Duan Z., Blau C.A. (2009) Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Cell Stem Cell 4(4):359-69.

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Stemolecule™ Sodium Butyrate

Sodium butyrate is the sodium salt of the short-chain fatty acid butyric acid¹. Butyrate is a metabolite of intestinal bacteria and a major energy source for gut epithelial cells, and is known to play a key role in the homeostasis of the gastrointestinal tract². The effects of sodium butyrate on cell volume regulation and chloride transport in the rat distal colon were first described by M. Diener et al. in 1997³. Sodium butyrate is a known inhibitor of histone deacetylases^4,5. The enhancement and suppression of various cytokines in stimulated human monocytes using sodium butyrate was first reported in 2000². In cultured mouse and human cells, sodium butyrate has been shown to inhibit both the mRNA and protein level of cyclin D1⁶.

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References

Kolthoff, I. M. et al., Quantitative Chemical Analysis, 4th ed., MacMillan Pub. (New York, NY: 1969).
Saemann, M. D. et al., FASEB J., 14(15):2380-2382 (2000).
Diener, M., and Scharrer, E., Biochem. Physiol. A Physiol., 118(2):375-379 (1997).
Kruh, J., Mol. Cell. Biochem. ,42(2):65-82 (1982).
Ware C.B., Wang L., Mecham B.H., Shen L., Nelson A.M., Bar M., Lamba D.A., Dauphin D.S., Buckingham B., Askari B., Lim R., Tewari M., Gartler S.M., Issa J.P., Pavlidis P., Duan Z., Blau C.A. (2009) Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Cell Stem Cell 4(4):359-69.
Lallemand, F. et al., Biochem. Biophys. Res. Commun., 229(1):163-169 (1996).

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For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Sodium Butyrate

Chemical Formula	C₄H₇NaO₂
Structure
Molecular Weight	111.1
CAS Number	156-54-7
PubChem Substance ID	24891873
Purity	>98% by titration
Formulation	White solid
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 4500 µl of DMSO to 5 mg of compound. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	4°C protected from light. Following reconstitution, store aliquots at -20°C
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of sodium butyrate was determined by HPLC analysis. The accurate mass and structure of sodium butyrate was determined by mass spectrometry and NMR, respectively. Cellular toxicity of sodium butyrate was tested by using HeLa and HEK293 cells.
References	Kolthoff, I. M. et al., Quantitative Chemical Analysis, 4th ed., MacMillan Pub. (New York, NY: 1969). Saemann, M. D. et al., FASEB J., 14(15):2380-2382 (2000). Diener, M., and Scharrer, E., Biochem. Physiol. A Physiol., 118(2):375-379 (1997). Kruh, J., Mol. Cell. Biochem. ,42(2):65-82 (1982). Ware C.B., Wang L., Mecham B.H., Shen L., Nelson A.M., Bar M., Lamba D.A., Dauphin D.S., Buckingham B., Askari B., Lim R., Tewari M., Gartler S.M., Issa J.P., Pavlidis P., Duan Z., Blau C.A. (2009) Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Cell Stem Cell 4(4):359-69. Lallemand, F. et al., Biochem. Biophys. Res. Commun., 229(1):163-169 (1996).