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Stemolecule™ SB431542

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SB431542 has been shown to stimulate proliferation, differentiation, and sheet formation of endothelial cells derived from embryonic stem cells via ALK receptor inhibition.1,2

1. Inman, G. J. et al., Mol. Pharmacol., 62:65-74 (2002).
2. Watabe T. et al., J. Cell Biol., 163(6):1303-1311 (2003).
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Structure
Stemolecule™ SB431542 chemical structure.
Structure
Now you can induce highly efficient neural conversion of human ES and iPS cells by using Stemfactor™ Noggin and Stemolecule™ SB431542!

Chambers et al.1 utilized a synergistic effect of Noggin and SB431542, two inhibitors of SMAD signaling, to effectively induce neural conversion from human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells. The efficiency of neural conversion by the dual inhibition is greater than 80% under adherent culture conditions. This discovery should overcome the drawbacks of traditional protocols used for neural conversion and facilitate the use of hES and hiPS cells for the field of regenerative medicine and disease modeling.

1. Chambers, S.M., Fasano, C. A., Papapetrou, E. P., Tomishima, M., Sadelain, M., and Studer, L. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 27(3), 275-280.

Stemolecule™ SB431542

Stemolecule™ SB431542 is an inhibitor of the TGF-β1 activin receptor-like kinases (ALKs). It is a selective and potent inhibitor of ALK-4, -5 and -7. SB431542 inhibits endogenous activin and TGF-β signaling without affecting more divergent BMP signaling utilizing ALK-1, -2, -3, and -61, 2. Watabe and colleagues (2003) reported that at a concentration of 1μM, SB431542 stimulates proliferation, differentiation, and sheet formation of endothelial cells derived from embryonic stem cells3.

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References

  1. Laping, N. J. et al., Mol. Pharmacol., 62:58-64 (2002).
  2. Inman, G. J. et al., Mol. Pharmacol., 62:65-74 (2002).
  3. Watabe T. et al., J. Cell Biol., 163(6):1303-1311 (2003).
  4. Tongxiang Lin, Rajesh Ambasudhan, Xu Yuan, Wenlin Li, Simon Hilcove, Ramzey Abujarour, Xiangyi Lin, Heung Sik Hahm, Ergeng Hao, Alberto Hayek & Sheng Ding. (2009) A chemical platform for improved induction of human iPSCs. Nat Meth 1393.

Related Products
Stemfactor™ Noggin, Human Recombinant (Cat. No. 03-0006)

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ SB431542

Chemical Formula C22H16N4O3
Alternate Name 4-(5-Benzol[1,3]dioxol-5-yl-4-pyrldin- 2-yl-1H-imidazol-2-yl)-benzamide hydrate 4-[4-(1,3-Benzodioxol-5-yl)-5-(2-pyridinyl)-1H- imidazol-2-yl]-benzamide hydrate 4-[4-(3,4-Methylenedioxyphenyl)-5-(2-pyridyl)- 1H-imidazol-2-yl]-benzamide hydrate
Structure [structure graphics]
Molecular Weight 384.4
CAS Number 301836-41-9 (anhydrous)
PubChem Substance ID 24724609
Purity >98.3% by HPLC analysis
Formulation White solid
Solubility Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 1301 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage 4°C protected from light. Following reconstitution, store aliquots at -20°C
Stability Stock solutions stable at -20°C for 6 months
Quality Control The purity of SB431542 was determined by HPLC analysis. The accurate mass and structure of SB431542 was determined by mass spectrometry and NMR, respectively. Cellular toxicity of PD173074 was tested by using HeLa and HEK293 cells.
References
  1. Laping, N. J. et al., Mol. Pharmacol., 62:58-64 (2002).
  2. Inman, G. J. et al., Mol. Pharmacol., 62:65-74 (2002).
  3. Watabe T. et al., J. Cell Biol., 163(6):1303-1311 (2003).
  4. Tongxiang Lin, Rajesh Ambasudhan, Xu Yuan, Wenlin Li, Simon Hilcove, Ramzey Abujarour, Xiangyi Lin, Heung Sik Hahm, Ergeng Hao, Alberto Hayek & Sheng Ding. (2009) A chemical platform for improved induction of human iPSCs. Nat Meth 1393.

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