Stemolecule™ Purmorphamine

Stemolecule™ Purmorphamine

Stemolecule™ Purmorphamine is a small molecule that promotes the differentiation of human and murine mesenchymal progenitor cells into osteoblasts^1,2. Purmorphamine's effect on differentiation of C3H10T1/2 cells was determined based on alkaline phosphatase expression, resulting in an EC₅₀ value of 1 μM¹. The mechanism of action study of Purmorphamine indicates that it is an agonist of Smoothened, a 7-transmembrane receptor of the hedgehog signaling pathway^3,4. Hedgehog signaling is involved in several cell differentiation processes and, recently, Fontaine and colleagues (2008) found that hedgehog signaling alters maturation of human mesenchymal stem cells into adipocytes⁵. Purmorphamine can be used also to replace sonic hedgehog for generation of motor neurons from human embryonic stem cells as described by Li et al. (2008)⁶.

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References

1. Wu, X. et al., J Am Chem Soc. 124:14520-14521 (2002).
2. Beloti, M. M. et al., Journal of Cell Biology International 29 (7):537-541 (2005).
3. Wu, X. et al., Chemistry and Biology 11:1229-1238 (2004).
4. Sinha, S. et al., Nature Chemical Biology 2(1):29-30 (2006).
5. Fontaine, C. et al., Stem Cells 26(4):1037-1046 (2008).
6. Li, X. J. et al., Stem Cells 26(4):886-893 (2008).

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Purmorphamine

Chemical Formula	C31H32N6O2
Alternate Name	2-(1-Naphthoxy)-6-(4-morpholinoanilino)- 9-cyclohexylpurine
Structure
Molecular Weight	520.6
CAS Number	483367-10-8
Purity	>98.6% by HPLC analysis
Formulation	Pale beige solid
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 960 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	4°C protected from light. Following reconstitution, store aliquots at -20°C
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of Purmorphamine was determined by HPLC analysis. The accurate mass and structure of Purmorphamine was determined by mass spectrometry and NMR, respectively. Cellular toxicity of PD173074 was tested by using HeLa and HEK293 cells.
References	Wu, X. et al., J Am Chem Soc. 124:14520-14521 (2002). Beloti, M. M. et al., Journal of Cell Biology International 29 (7):537-541 (2005). Wu, X. et al., Chemistry and Biology 11:1229-1238 (2004). Sinha, S. et al., Nature Chemical Biology 2(1):29-30 (2006). Fontaine, C. et al., Stem Cells 26(4):1037-1046 (2008). Li, X. J. et al., Stem Cells 26(4):886-893 (2008).

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