Stemolecule™ SC1 (Pluripotin)

Catalog #
04-0011
Size
1 mg
Price (USD)
$99.00
Qty
Order Offline
Tel: 877.228.9783
Email: orders@stemgent.com
Fax: 617-494-0398

Product Overview

SC1 (Pluripotin) was identified in a cell-based screen of a chemical library for small molecules that regulate the mouse embryonic stem (ES) cell self-renewal1. It was shown that SC1 is sufficient to sustain long-term self-renewal of mouse ES cells in an undifferentiated state in the absence of LIF, feeder cells, or serum. SC1 acts through dual inhibition of extracellular signal-regulated kinase 1 (ERK1) and Ras GTPase-activating protein (Ras-GAP). Inactivation of ERK1 blocks differentiation, whereas inhibition of Ras-GAP activates Ras and PI3K, promoting self-renewal of mouse ES cells. Mouse ES cells maintained in the presence of SC1 retain their ability to differentiate into cells representing all three germ layers in vitro, form chimeric mouse, and contribute to germ line in vivo.

References

  1. Chen, S., Do, J. T., Zhang, Q., Yao, S., Yan, F., Peters, E. C., Schöler, H. R., Schultz, P. G., and Ding, S. (2006) Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci USA 103(46):17266-17271.
Picture1
The use of SC1 (Pluripotin) to support mESC self-renewal in the absence of LIF

Picture1 04-0011_stemolecule™_sc1_pluripotin_

For research use only. Not for use in diagnostic procedures. Not for resale.

Product Specifications

Chemical Formula C27H25F3N8O2
Alternate Name Pluripotin
Structure [structure graphics]
Molecular Weight 550.54
Purity >98.5%
Formulation White solid
Solubility Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 624 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage 4°C protected from light. Reconstitute by using DMSO to make 100 mM. Following reconstitution, store aliquots at -20°C.
Stability Stock solutions stable at -20°C for 6 months
Quality Control The purity of SC1 was determined by HPLC analysis. The accurate mass and structure of SC1 was determined by mass spectrometry and NMR, respectively. Cellular toxicity of SC1 was tested on mouse ES cells, HeLa and HEK293 cells.

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