Stemolecule™ SC1 (Pluripotin)

Stemolecule™ SC1 (Pluripotin)

SC1 (Pluripotin) was identified in a cell-based screen of a chemical library for small molecules that regulate the mouse embryonic stem (ES) cell self-renewal¹. It was shown that SC1 is sufficient to sustain long-term self-renewal of mouse ES cells in an undifferentiated state in the absence of LIF, feeder cells, or serum. SC1 acts through dual inhibition of extracellular signal-regulated kinase 1 (ERK1) and Ras GTPase-activating protein (Ras-GAP). Inactivation of ERK1 blocks differentiation, whereas inhibition of Ras-GAP activates Ras and PI3K, promoting self-renewal of mouse ES cells. Mouse ES cells maintained in the presence of SC1 retain their ability to differentiate into cells representing all three germ layers in vitro, form chimeric mouse, and contribute to germ line in vivo.

References

1. Chen, S., Do, J. T., Zhang, Q., Yao, S., Yan, F., Peters, E. C., Schöler, H. R., Schultz, P. G., and Ding, S. (2006) Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci USA 103(46):17266-17271.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ SC1 (Pluripotin)

Chemical Formula	C27H25F3N8O2
Alternate Name	Pluripotin
Structure
Molecular Weight	550.54
Purity	>98.5%
Formulation	White solid
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 624 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	4°C protected from light. Reconstitute by using DMSO to make 100 mM. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of SC1 was determined by HPLC analysis. The accurate mass and structure of SC1 was determined by mass spectrometry and NMR, respectively. Cellular toxicity of SC1 was tested on mouse ES cells, HeLa and HEK293 cells.
References	Chen, S., Do, J. T., Zhang, Q., Yao, S., Yan, F., Peters, E. C., Schöler, H. R., Schultz, P. G., and Ding, S. (2006) Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci USA 103(46):17266-17271.

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MSDS

• Material Safety Data Sheet

Protocols

• General Protocol for Immunocytochemistry

Application Notes

The use of SC1 (Pluripotin) to support mESC self-renewal in the absence of LIF

Wiki

• Small Molecules
• Stemolecule™ SC1 (Pluripotin)