Stemolecule™ Y27632

Stemolecule™ Y27632

Y27632 is a Rho-associated kinase ROCK inhibitor¹. Y27632 has recently been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency². This inhibitor has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors³.

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References

1. Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000). Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57, 976-983.
2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J. B., Nishikawa, S., Nishikawa, S., Muguruma, and K., Sasai, Y. (2007). A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25, 681-686.
3. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008). Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J. Neurosci Res 86, 270-280.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Y27632

Alternate Name	(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride
Chemical Formula	C14H23Cl2N3O
Structure
Molecular Weight	320.3
CAS Number	146986-50-7
Purity	>97.5% by HPLC analysis
Formulation	White solid
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 182 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of Y27632 was determined by HPLC analysis. The accurate mass and structure of Y27632 was determined by mass spectrometry and NMR, respectively. Cellular toxicity of Y27632 was tested by using HeLa and HEK293 cells.
References	Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000). Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57, 976-983. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J. B., Nishikawa, S., Nishikawa, S., Muguruma, and K., Sasai, Y. (2007). A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25, 681-686. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008). Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J. Neurosci Res 86, 270-280.

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