Stemolecule™ R(+)Bay K 8644

Stemolecule™ R(+)Bay K 8644

R(+)Bay K 8644 is an L-type Ca²⁺ channel activator^1,2. This molecule has recently been demonstrated to enhance reprogramming efficiency. When only two transcription factors (Oct4 and Klf4) are introduced into mouse embryonic fibroblasts (MEFs), and when used in combination with a histone methyltransferase G9a inhibitor BIX01294, R(+)Bay K 8644 enhances the efficiency of iPS cell generation significantly³.

View Poster

References

1. Hess, P., Lansman, J. B., and Tsien, R. W. (1984). Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists. Nature 311(5986), 538-544.
2. Nowycky, M. C., Fox, A. P., and Tsien, R. W. (1985). Long-opening mode of gating of neuronal calcium channels and its promotion by the dihydropyridine calcium agonist Bay K 8644. Proc Natl Acad Sci USA 82(7), 2178-2182.
3. Shi, Y., Desponts, C., Do, J. T., Hahm, H. S., Schoeler, H. R., and Ding, S. (2008). Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3(5), 568-574.

Related Products
Stemolecule™ BIX01294 (Cat. No. 04-0002)

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ R(+)Bay K 8644

Alternate Name	(4R)-1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-trifluoromethyl)phenyl]-3-pyridinecarboxylic acid methyl ester
Chemical Formula	C16H15F3N2O4 1/2 H2O
Structure
Molecular Weight	356.31
CAS Number	98791-67-4
Purity	>97.8%
Formulation	Yellow solid
Storage and handling	Soluble in DMSO (100 mM) or ethanol (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 1403 µl of DMSO to the entire contents of the vial. Store at 4°C. Protect from light. Reconstitute by diluting with DMSO or ethanol to a 100 mM solution. Following reconstitution, store aliquots at -20°C. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of R(+)Bay K 8644 was determined by HPLC analysis. The accurate mass and structure of R(+)Bay K 8644 was determined by mass spectrometry and NMR, respectively. Cellular toxicity of R(+)Bay K 8644 was tested using HeLa and HEK293 cells.
References	Hess, P., Lansman, J. B., and Tsien, R. W. (1984). Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists. Nature 311(5986), 538-544. Nowycky, M. C., Fox, A. P., and Tsien, R. W. (1985). Long-opening mode of gating of neuronal calcium channels and its promotion by the dihydropyridine calcium agonist Bay K 8644. Proc Natl Acad Sci USA 82(7), 2178-2182. Shi, Y., Desponts, C., Do, J. T., Hahm, H. S., Schoeler, H. R., and Ding, S. (2008). Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3(5), 568-574.

Download PDF

MSDS

• Material Safety Data Sheet

Wiki

• Small Molecules
• Stemolecule™ R(+)Bay K 8644