Stemolecule™ ALK5 Inhibitor

Stemolecule™ ALK5 Inhibitor

Stemolecule™ ALK5 Inhibitor is a selective and ATP-competitive inhibitor of a TGF-β family type I receptor, activin receptor-like kinase (ALK5)¹. Inhibition of TGF-β signaling increased the efficiency of reprogramming of rat liver progenitor cells and IMR90 human fibroblast cells to mouse embryonic stem (ES) cell-like induced pluripotent stem (iPS) cells, as well as maintained these cells in an undifferentiated state for over 30 passages². The mouse ES cell culture condition together with inactivation of TGF-β signaling provided a robust culturing method of reprogrammed rat and human iPS cells². This study indicates the significant role of TGF-β signaling in regulating ES cell fate determination, and provides a novel method for culture of rat and human iPS cells.

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References

1. Gellibert, F., Woolven, J., Fouchet, M. H., Mathews, N., Goodland, H., Lovegrove, V., Laroze, A., Nguyen, V. L., Sautet, S., Wang, R., Janson, C., Smith, W., Krysa, G., Boullay, V., De Gouville, A. C., Huet, S., and Hartley, D. (2004) Identification of 1, 5-naphthyridine derivatives as a novel series of potent and selective TGF-beta type I receptor inhibitors. J Med Chem 47, 4494-506.
2. Li, W., Wei, W., Zhu, S., Zhu, J., Shi, Y., Lin, T., Hao, E., Hayek, A., Deng, H., and Ding, S. (2008) Generation of Rat and Human Induced Pluripotent Stem Cells by Combining Genetic Reprogramming and Chemical Inhibitors. Cell Stem Cell 4, 16-19.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ ALK5 Inhibitor

Alternate Name	2-(3-(6-methylpyridine-2-yl)-1H-pyrazol-4-yl)-1,5-naphthyridine
Chemical Formula	C17H13N5
Structure
Molecular Weight	287.32
CAS Number	NA
Purity	>99%
Formulation	Yellow solid
Solubility	Soluble in DMSO (100 mM) or methanol (17 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 384 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C
Stability	Stock solutions stable at -20°C for 6 months
Quality Control	The purity of Stemolecule ALK5 Inhibitor was determined by HPLC analysis. The accurate mass and structure of Stemolecule ALK5 Inhibitor was determined by mass spectrometry and NMR, respectively. Cellular toxicity of ALK5 Inhibitor was tested on HeLa and HEK293 cells.
References	Gellibert, F., Woolven, J., Fouchet, M. H., Mathews, N., Goodland, H., Lovegrove, V., Laroze, A., Nguyen, V. L., Sautet, S., Wang, R., Janson, C., Smith, W., Krysa, G., Boullay, V., De Gouville, A. C., Huet, S., and Hartley, D. (2004) Identification of 1, 5-naphthyridine derivatives as a novel series of potent and selective TGF-beta type I receptor inhibitors. J Med Chem 47, 4494-506. Li, W., Wei, W., Zhu, S., Zhu, J., Shi, Y., Lin, T., Hao, E., Hayek, A., Deng, H., and Ding, S. (2008) Generation of Rat and Human Induced Pluripotent Stem Cells by Combining Genetic Reprogramming and Chemical Inhibitors. Cell Stem Cell 4, 16-19.

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