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Stemgent® Dox Inducible Reprogramming Lentivirus Set: Human OKSM (conc)
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Product Overview
Each doxycycline-inducible lentivirus in this set is capable of expressing one of four human transcription factors (Oct4, Klf4, Sox2 or c-Myc) under the control of the doxycycline (DOX)-inducible tetO operator when transduced into mammalian cells. Each unique virus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of these four transcription factors, along with the reverse tetracycline transcriptional activator (rtTA; not included in the set, also see Related Products), has been shown to reprogram human adult fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell1.
The Stemgent® iPSC Generation DOX Inducible Human TF Lentivirus Set: OKSM (Concentrated) was developed in the lab of Rudolf Jaenisch, M.D., at the Whitehead Institute at MIT1. Dr. Jaenisch is a recognized leader in the study of epigenetic regulation of gene expression with numerous publications focused on ES and iPS cellular mechanisms and techniques.
References
- Hockemeyer, D., Soldner, F., Cook, E.G., Gao, Q., Mitalipova, M., and Jaenisch, R. (2008) A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell 3,346-353.
Immunocytochemistry (ICC) analysis 48 hours post-doxycycline (Dox) induction to monitor transduction efficiency (200x magnification)
For research use only. Not for use in diagnostic procedures. Not for resale.
Product Specifications
| Quality Control |
Viral concentration determined by p24 (capsid antigen) ELISA assay. Transcription factor protein expression verified by immunocytochemistry analysis of transduced human foreskin fibroblasts.
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| Size |
4 x 100 μl
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| Lot Number |
1136
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| Vial Contents |
100 μl Dox Inducible hOct4 virus (Cat. No. 07-0035, Lot 1131) concentration: 1990 ng/ml
100 μl Dox Inducible hKlf4 virus (Cat. No. 07-0037, Lot 1133) concentration: 3490 ng/ml
100 μl Dox Inducible hSox2 virus (Cat. No. 07-0036, Lot 1132) concentration: 409 ng/ml
100 μl Dox Inducible hc-Myc virus (Cat. No. 07-0038, Lot 1134) concentration: 2020 ng/ml
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| Sterility |
Tested to be free of bacteria. TSB (tryptic soy broth) was inoculated with each virus and cultured for 4 days at 30°C and 3 days at room temperature to assay for bacterial growth.
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| Storage and Stability |
Store at -80°C for up to 9 months. Keep frozen until use. Avoid freeze thaw cycles.
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| Related Products |
rtTA is required for use with this product. Stemgent® iPSC Generation Dox Inducible Human TF Lentivirus Set: OKSM + RG (Concentrated) (Cat. No. 00-0037) includes the four factors along with the rtTA and GFP lentiviruses.
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| Use Restriction |
For restrictions related to the use of this product please refer to the accompanying Restricted Use License.
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Technical Documents
Specifications
MSDS
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