Spec Sheet

Stemgent® Dox Inducible Reprogramming Lentivirus Set: Human OKSM (conc)

This Product has been Discontinued

Catalog #	Size	Price (USD)	Quantity
00-0012	4 x 100 μl	$1999.00	Qty:

The Stemgent® iPSC Generation Dox Inducible Human TF Lentivirus Set: OKSM (Concentrated) provides an affordable inducible method for generating iPS cells. The set includes hOct4, hSox2, hc-Myc, and hKlf4 transcription factors as prepackaged lentivirus particles tested and proven to reprogram human fibroblast cells.

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Immunocytochemistry (ICC) analysis 48 hours post-doxycycline (Dox) induction to monitor transduction efficiency (200x magnification)

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General
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Technical Documents

Stemgent® Dox Inducible Reprogramming Lentivirus Set: Human OKSM (conc)

Each doxycycline-inducible lentivirus in this set is capable of expressing one of four human transcription factors (Oct4, Klf4, Sox2 or c-Myc) under the control of the doxycycline (DOX)-inducible tetO operator when transduced into mammalian cells. Each unique virus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of these four transcription factors, along with the reverse tetracycline transcriptional activator (rtTA; not included in the set, also see Related Products), has been shown to reprogram human adult fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell¹.

The Stemgent® iPSC Generation DOX Inducible Human TF Lentivirus Set: OKSM (Concentrated) was developed in the lab of Rudolf Jaenisch, M.D., at the Whitehead Institute at MIT¹. Dr. Jaenisch is a recognized leader in the study of epigenetic regulation of gene expression with numerous publications focused on ES and iPS cellular mechanisms and techniques.

References

Hockemeyer, D., Soldner, F., Cook, E.G., Gao, Q., Mitalipova, M., and Jaenisch, R. (2008) A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell 3,346-353.

Related Products
Stemolecule™ Doxycycline hyclate (Cat. No. 04-0016)
Stemgent^® Dox Inducible Reprogramming Lentivirus: Human Oct4 (conc) (Cat. No. ST070035-100ul)
Stemgent^® Dox Inducible Reprogramming Lentivirus: Human Sox2 (conc) (Cat. No. ST070036-100ul)
Stemgent^® Dox Inducible Reprogramming Lentivirus: Human Klf4 (conc) (Cat. No. ST070037-100ul)
Stemgent^® Dox Inducible Reprogramming Lentivirus: Human c-Myc (conc) (Cat. No. ST070038-100ul)
Stemgent^® Dox Inducible Reprogramming Lentivirus Set: Human OKSM (conc.) (Cat. No. ST000037-1Set)

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemgent® Dox Inducible Reprogramming Lentivirus Set: Human OKSM (conc)

Quality Control	Viral concentration determined by p24 (capsid antigen) ELISA assay. Transcription factor protein expression verified by immunocytochemistry analysis of transduced human foreskin fibroblasts.
Size	4 x 100 μl
Lot Number	1136
Vial Contents	100 μl Dox Inducible hOct4 virus (Cat. No. 07-0035, Lot 1131) concentration: 1990 ng/ml 100 μl Dox Inducible hKlf4 virus (Cat. No. 07-0037, Lot 1133) concentration: 3490 ng/ml 100 μl Dox Inducible hSox2 virus (Cat. No. 07-0036, Lot 1132) concentration: 409 ng/ml 100 μl Dox Inducible hc-Myc virus (Cat. No. 07-0038, Lot 1134) concentration: 2020 ng/ml
Sterility	Tested to be free of bacteria. TSB (tryptic soy broth) was inoculated with each virus and cultured for 4 days at 30°C and 3 days at room temperature to assay for bacterial growth.
Storage and Stability	Store at -80°C for up to 9 months. Keep frozen until use. Avoid freeze thaw cycles.
Related Products	rtTA is required for use with this product. Stemgent® iPSC Generation Dox Inducible Human TF Lentivirus Set: OKSM + RG (Concentrated) (Cat. No. 00-0037) includes the four factors along with the rtTA and GFP lentiviruses.
References	Hockemeyer, D., Soldner, F., Cook, E.G., Gao, Q., Mitalipova, M., and Jaenisch, R. (2008) A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell 3,346-353.
Use Restriction	For restrictions related to the use of this product please refer to the accompanying Restricted Use License.