Spec Sheet Application_tested

Stemgent® Dox Inducible Reprogramming Lentivirus Kit: Mouse OKSM

Catalog # Size Price (USD) Quantity
00-0024
$2499.00 Qty:

The Stemgent® Mouse iPSC Generation Kit is fully validated and designed to simplify and facilitate the production of mouse induced pluripotent stem cells. The iPSC Generation Dox Inducible Mouse TF Lentivirus Set: OKSM + RG includes each of the four mouse transcription factors (Oct4, Klf4, Sox2, and c-Myc) and is able to reprogram the Oct4-neo mouse embryonic fibroblasts (MEFs) (P2) to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell. The expression of these four factors, along with the reverse tetracycline transcriptional activator (rtTA), has been shown to reprogram MEFs to iPS cells. In addition, the Mouse ES/iPS Cell Characterization Set contains all the antibodies (SSEA-1, Nanog, and Oct4) and the Alkaline Phosphatase Staining Kit to fully characterize mouse iPS and ES cells.

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ICC Analysis 48 Hours Post-Dox Induction to Monitor Transduction Efficiency of the Lentiviruses in the Dox Inducible Reprogramming Lentivirus Set: Mouse OKSM
Icc_analysis_00-0021 Cell_morphologies_00-0021 Analysis_of_ips_colonies_00-0021

Stemgent® Dox Inducible Reprogramming Lentivirus Kit: Mouse OKSM

This Mouse iPSC Generation Kit is fully validated and designed to simplify and facilitate the production of mouse induced pluripotent stem cells. The iPSC Generation Dox Inducible Mouse TF Lentivirus Set: OKSM + RG includes each of the four mouse transcription factors (Oct4, Klf4, Sox2, and c-Myc) and is able to reprogram the provided Oct4-neo mouse embryonic fibroblasts (MEFs) (P2) to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell. The expression of these four factors, along with the reverse tetracycline transcriptional activator (rtTA), has been shown to reprogram MEFs to iPS cells. In addition, the Mouse ES/iPS Cell Characterization Set contains all the antibodies (SSEA-1, Nanog, and Oct4) and the Alkaline Phosphatase Staining Kit to fully characterize mouse iPS and ES cells.

Related Products
Stemgent® Oct4-neo MEF (P2) (Cat. No. 08-0014)
Stemgent® Mouse ES/iPS Cell Characterization Set (Cat. No. 00-0016)
Stemolecule™ Doxycycline hyclate (Cat. No. 04-0016)
Stemgent® Dox Inducible Reprogramming Lentivirus Set: Mouse OKSM (Cat. No. ST000021-1Set)

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemgent® Dox Inducible Reprogramming Lentivirus Kit: Mouse OKSM

Size 1 Kit with 4 components
Lot Number 1263
Quantity / Size 1 Kit with 4 components
Kit Contents 1. Stemgent® iPSC Generation Dox Inducible Mouse TF Lentivirus Set: OKSM + RG (Cat. No. 00-0021, Lot 1170)
2. Stemgent® Oct4-neo MEF (P2) (Cat. No. 08-0014, Lot 1072)
3. Stemgent® Mouse ES/iPS Cell Characterization Set (Cat. No. 00-0016, Lot 1162)
4. Stemgent® Doxycycline (Cat. No. 04-0016, Lot 1203)
Quality Control Please see individual product Spec Sheets.
Storage and Stability It is important to store the components of the Stemgent® Mouse iPSC Generation Kit II under their proper conditions.
1. Remove Oct4-neo MEF (P2) cells and store in liquid nitrogen for up to 6 months.
2. Remove the iPSC Generation Dox Inducible Mouse TF Lentivirus Set and store at -80°C for up to 9 months. Avoid multiple freeze and thaw cycles.
3. Store the Mouse ES/iPS Cell Characterization Set at 4°C for up to 6 months. Do not freeze.
4. Remove the Doxycycline and store at -20°C for up to 6 months.
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Use Restriction For restrictions related to the use of this product please refer to the accompanying Restricted Use License.

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