Stemolecule™ Dexamethasone

Stemolecule™ Dexamethasone

Stemolecule™ Dexamethasone is a glucocorticoid receptor agonist known to affect inflammatory responses, cellular proliferation, and differentiation in target tissues¹. Dexamethasone in combination with other small molecules, such as ascorbic acid, β-glycerophosphate, isobutylmethylxanthine or peroxisome proliferator activated receptor-gamma agonists has been widely used to modulate osteogenesis or adipogenesis of mesenchymal stem cells under specific conditions^2,3. Differentiation of cord blood stem cell derived-dendritic cell (DC) precursors into immature DCs is inhibited by Dexamethasone⁴. Transdifferentiation of pancreatic cells into hepatocytes can be accomplished with dexamethasone treatment⁵.

References

1. Cole, T.J. (2006) Glucocorticoid action and the development of selective glucocorticoid receptor ligands. Biotechnol Annu Rev 12: 269-300.
2. Grigoriadis, A.E., Heersche, J.N., Aubin, J.E. (1988) Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone. J Cell Biol 106: 2139-51.
3. Jaiswal, N., Haynesworth, S.E., Caplan, A.I., Bruder, S.P. (1997) Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 64: 295-312.
4. Mainali, E.S., Tew, J.G. (2004) Dexamethasone selectively inhibits differentiation of cord blood stem cell derived-dendritic cell (DC) precursors into immature DCs. Cell Immunol 232: 127-36.
5. Shen, C.N., Slack, J.M., Tosh, D. (2000) Molecular basis of transdifferentiation of pancreas to liver. Nat Cell Biol 2: 879-87.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Dexamethasone

Alternate Names	(8S,9R,10S,11S,13S,14S,16R,17R)-9-Fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-6,7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-3-one; DEX; Dexamethazone; DXM
Chemical Formula	C22H29FO5
Structure
Molecular Weight	392.46
CAS Number	50-02-2
Purity	99%
Formulation	White solid
Solubility	Soluble in DMSO (100 mM) or ethanol (40 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 1274 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months.
Quality control	The purity of Dexamethasone was determined by HPLC analysis. The accurate mass and structure of Dexamethasone was determined by mass spectrometry and NMR, respectively. Cellular toxicity of Dexamethasone was tested on mouse embryonic stem cells.
References	Cole, T.J. (2006) Glucocorticoid action and the development of selective glucocorticoid receptor ligands. Biotechnol Annu Rev 12: 269-300. Grigoriadis, A.E., Heersche, J.N., Aubin, J.E. (1988) Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone. J Cell Biol 106: 2139-51. Jaiswal, N., Haynesworth, S.E., Caplan, A.I., Bruder, S.P. (1997) Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 64: 295-312. Mainali, E.S., Tew, J.G. (2004) Dexamethasone selectively inhibits differentiation of cord blood stem cell derived-dendritic cell (DC) precursors into immature DCs. Cell Immunol 232: 127-36. Shen, C.N., Slack, J.M., Tosh, D. (2000) Molecular basis of transdifferentiation of pancreas to liver. Nat Cell Biol 2: 879-87.

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