Product Overview

Stemolecule™ IDE-2 was identified in a high throughput screen as an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells¹. IDE-2 is able to generate Sox17⁺/FoxA2⁺ cells to an equal or greater efficiency than that of protein (i.e. Activin A or Nodal) inducers of definitive endoderm in mouse or human ES cell cultures¹. It has an EC₅₀ of 223 nM and functions in part via activation of TGF-β signaling, as evident by Smad2 phosphorylation¹. Endoderm chemically induced by IDE-2 can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos¹.

References

1. Borowiak, M., Maehr, R., Chen, S., Chen, A.E., Tang, W., Fox, J.L., Schreiber, S.L., Melton, D.A. (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4: 348-58.