Stemolecule™ IDE-2

Stemolecule™ IDE-2

Stemolecule™ IDE-2 was identified in a high throughput screen as an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells¹. IDE-2 is able to generate Sox17⁺/FoxA2⁺ cells to an equal or greater efficiency than that of protein (i.e. Activin A or Nodal) inducers of definitive endoderm in mouse or human ES cell cultures¹. It has an EC₅₀ of 223 nM and functions in part via activation of TGF-β signaling, as evident by Smad2 phosphorylation¹. Endoderm chemically induced by IDE-2 can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos¹.

References

1. Borowiak, M., Maehr, R., Chen, S., Chen, A.E., Tang, W., Fox, J.L., Schreiber, S.L., Melton, D.A. (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4: 348-58.

Related Products
Stemolecule™ IDE-1 (Cat. No. 04-0026)

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ IDE-2

Size / Quantity	2 mg
Alternate Name	7-(2-cyclopentylidenehydrazinyl)-7-oxoheptanoic acid, Induce definitive endoderm-2
CAS Number	N/A
Chemical Formula	C12H20N2O3
Structure
Formulation	White Powder
Molecular Weight	240.30
Purity	>99%
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 832 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months.
Quality Control	The purity of IDE-2 was determined by HPLC analysis. The accurate mass and structure of IDE-2 were determined by mass spectrometry and NMR, respectively. Cellular toxicity of IDE-2 was tested on mouse ES cells.
References	Borowiak, M., Maehr, R., Chen, S., Chen, A.E., Tang, W., Fox, J.L., Schreiber, S.L., Melton, D.A. (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4: 348-58.
Related Products	Stemolecule™ IDE-1 (04-0026)

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