Stemolecule™ Rock I Inhibitor

Stemolecule™ Rock I Inhibitor

Stemolecule^TM Rock I Inhibitor is a specific inhibitor for type 1 Rho-associated, coiled-coil containing protein kinase (Rock). Rocks are serine/threonine-specific protein kinases which play a crucial role in organization of the actin cytoskeleton¹. Rock inhibitors have been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency^1,2.

References

1. Olson, M.F. (2008) Applications for ROCK kinase inhibition. Curr Opin Cell Biol 20: 242-8.
2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Muguruma, K., Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-6.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Rock I Inhibitor

Size / Quantity	2 mg
Alternate Name	(+)-4-[1(R)-Aminoethyl]-N-(1H-pyrrolo[2,3-b]pyridine-4-yl)benzamide dihydrochloride hydrate, RKI-983, SNJ-1656, Y-39983
CAS Number	203911-26-6
Chemical Formula	C16H21Cl2N4O2.5
Structure
Formulation	White powder
Molecular Weight	380.27
Purity	99.5%
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 526 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store lypholized powder at either 4°C or -20°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months.
Quality Control	The purity of Rock I Inhibitor was determined by HPLC analysis. The accurate mass and structure of Rock I Inhibitor were determined by mass spectrometry and NMR, respectively. Cellular toxicity of Rock I Inhibitor was tested on mouse ES cells.
References	Olson, M.F. (2008) Applications for ROCK kinase inhibition. Curr Opin Cell Biol 20: 242-8. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Muguruma, K., Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-6.
Notice to Purchaser	Purchaser represents and warrants that it will use Stemolecule™ Rock I Inhibitor purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.

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