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Product Overview
StemoleculeTM ROCK I Inhibitor is a specific inhibitor for type 1 Rho-associated, coiled-coil containing protein kinase (ROCK). Rocks are serine/threonine-specific protein kinases which play a crucial role in organization of the actin cytoskeleton1. ROCK inhibitors have been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency1,2.
References
- Olson, M.F. (2008) Applications for ROCK kinase inhibition. Curr Opin Cell Biol 20: 242-8.
- Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Muguruma, K., Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-6.
For research use only. Not for use in diagnostic procedures. Not for resale.
Product Specifications
| Structure |
![[structure graphics]](http://www.stemgent.com/products/04-0029/images/structure.jpg)
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| Alternate Name |
(+)-4-[1(R)-Aminoethyl]-N-(1H-pyrrolo[2,3-b]pyridine-4-yl)benzamide dihydrochloride hydrate, RKI-983, SNJ-1656, Y-39983
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| CAS Number |
203911-26-6
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| Chemical Formula |
C16H21Cl2N4O2.5
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| Formulation |
White powder
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| Molecular Weight |
380.27
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| Purity |
99.5%
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| Solubility |
Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 526 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
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| Storage |
Store lypholized powder at either 4°C or -20°C. Protect from light. Following reconstitution, store aliquots at -20°C.
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| Stability |
Stock solutions stable at -20°C for 6 months.
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| Quality Control |
The purity of ROCK I Inhibitor was determined by HPLC analysis. The accurate mass and structure of Rock I Inhibitor were determined by mass spectrometry and NMR, respectively. Cellular toxicity of Rock I Inhibitor was tested on mouse ES cells.
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Technical Documents
Specifications
MSDS
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