Stemolecule™ Rock II Inhibitor

Stemolecule™ Rock II Inhibitor

Stemolecule^TM Rock II Inhibitor is a specific inhibitor for type 2 Rho-associated, coiled-coil containing protein kinase (Rock)¹. Rocks are serine/threonine-specific protein kinases which play a crucial role in organization of the actin cytoskeleton². Rock inhibitors have been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency^2,3.

References

1. Feng, Y., Yin, Y., Weiser, A., Griffin, E., Cameron, M.D., Lin, L., Ruiz, C., Schurer, S.C., Inoue, T., Rao, P.V., Schroter, T., Lograsso, P. (2008) Discovery of substituted 4-(pyrazol-4-yl)-phenylbenzodioxane-2-carboxamides as potent and highly selective Rho kinase (ROCK-II) inhibitors. J Med Chem 51: 6642-5.
2. Olson, M.F. (2008) Applications for ROCK kinase inhibition. Curr Opin Cell Biol 20: 242-8.
3. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Muguruma, K., Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-6.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Rock II Inhibitor

Size / Quantity	2 mg
Alternate Name	N-(2-(2-(dimethylamino)ethoxy)-4-(1H-pyrazol-4-yl)phenyl)-2,3-dihydrobenzo[b][1,4]dioxine-2-carboxamide
CAS Number	N/A
Chemical Formula	C22H24N4O4
Structure
Formulation	White powder
Molecular Weight	408.45
Purity	99%
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 490 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store lypholized powder at either 4°C or -20°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months.
Quality Control	The purity of Rock II Inhibitor was determined by HPLC analysis. The accurate mass and structure of Rock II Inhibitor was determined by mass spectrometry and NMR, respectively. Cellular toxicity of Rock II Inhibitor was tested on mouse ES cells.
References	Feng, Y., Yin, Y., Weiser, A., Griffin, E., Cameron, M.D., Lin, L., Ruiz, C., Schurer, S.C., Inoue, T., Rao, P.V., Schroter, T., Lograsso, P. (2008) Discovery of substituted 4-(pyrazol-4-yl)-phenylbenzodioxane-2-carboxamides as potent and highly selective Rho kinase (ROCK-II) inhibitors. J Med Chem 51: 6642-5. Olson, M.F. (2008) Applications for ROCK kinase inhibition. Curr Opin Cell Biol 20: 242-8. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Muguruma, K., Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-6.
Notice to Purchaser	Purchaser represents and warrants that it will use Stemolecule™ Rock II Inhibitor purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.

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