Stemolecule™ Tranylcypromine hydrochloride

Stemolecule™ Tranylcypromine hydrochloride

Stemolecule™ Tranylcypromine hydrochloride is a non-hydrazine monoamine oxidase inhibitor commonly used as an antidepressant drug¹. BHC110/LSD1 is a histone H3 lysine 4 demethylation enzyme with close homology to monoamine oxidases². Nonselective monoamine oxidase inhibitors potently inhibit nucleosomal demethylation of histone H3 lysine. Tranylcypromine has been shown to inhibit BHC110/LSD1 activity (IC50 < 2 μM). Treatment of P19 embryonal carcinoma cells with tranylcypromine resulted in global increase in H3K4 methylation as well as transcriptional derepression of the pluripotent stem cell marker Oct4³.

References

1. Frieling, H., Bleich, S. (2006) Tranylcypromine: new perspectives on an "old" drug. Eur Arch Psychiatry Clin Neurosci 256: 268-73.
2. Metzger, E., Wissmann, M., Yin, N., Muller, J.M., Schneider, R., Peters, A.H., Gunther, T., Buettner, R., Schule, R. (2005) LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription. Nature 437: 436-9.
3. Lee, M.G., Wynder, C., Schmidt, D.M., McCafferty, D.G., Shiekhattar, R. (2006) Histone H3 lysine 4 demethylation is a target of nonselective antidepressive medications. Chem Biol 13: 563-7.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Tranylcypromine hydrochloride

Alternate Names	Dl-Tranylcypromine, Parnate, Transamine, (1R,2S)-2-phenylcyclopropanamine, trans-2-Phenylcyclopropylamine hydrochloride
Chemical Formula	C9H11N·HCl
Structure
Molecular Weight	169.65
CAS Number	1986-47-6
Purity	>97%
Formulation	White to off-white powder
Solubility	Soluble in DMSO (100 mM), water (29 mM), or ethanol (59 mM). For a 20 mM concentrated stock solution, reconstitute the compound by adding 2946 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months.
Quality Control	The purity of Tranylcypromine hydrochloride was determined by HPLC analysis. The accurate mass and structure of Tranylcypromine hydrochloride was determined by mass spectrometry and NMR, respectively. Cellular toxicity of Tranylcypromine hydrochloride was tested on mouse embryonic stem cells.
References	Frieling, H., Bleich, S. (2006) Tranylcypromine: new perspectives on an "old" drug. Eur Arch Psychiatry Clin Neurosci 256: 268-73. Metzger, E., Wissmann, M., Yin, N., Muller, J.M., Schneider, R., Peters, A.H., Gunther, T., Buettner, R., Schule, R. (2005) LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription. Nature 437: 436-9. Lee, M.G., Wynder, C., Schmidt, D.M., McCafferty, D.G., Shiekhattar, R. (2006) Histone H3 lysine 4 demethylation is a target of nonselective antidepressive medications. Chem Biol 13: 563-7.

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