Spec Sheet

Stemolecule™ Wnt Inhibitor IWP-3

Catalog #	Size	Price (USD)	Quantity
04-0035	2 mg	$99.00	Qty:

Stemolecule™ Wnt Inhibitor IWP-3 is an antagonist of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity¹. It blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin.

Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., Lum, L. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-7.

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Stemolecule™ Wnt Inhibitor IWP-3

Stemolecule™ Wnt Inhibitor IWP-3 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway¹. Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity¹. It has an IC₅₀ of 40 nM and blocks phosporylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin¹.

References

Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., Lum, L. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-7.

Related Products
Stemolecule™ Wnt Inhibitor IWP-4 (Cat. No. 04-0036)
Stemolecule™ Wnt Inhibitor IWP-2 (Cat. No. 04-0034)

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Wnt Inhibitor IWP-3

Size / Quantity	2 mg
Lot Number	1305
Alternate Name	2-(3-(4-fluorophenyl)-3,4,6,7-tetrahydro-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide
Chemical Formula	C₂₂H₁₇FN₄O₂S₃
Structure
Molecular Weight	484.59
CAS Number	N/A
Purity	>99%
Formulation	Light yellow solid
Solubility	Soluble in DMSO (100 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 413 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability	Stock solutions stable at -20°C for 6 months.
Quality control	The purity of Wnt Inhibitor IWP-3 was determined by HPLC analysis. The accurate mass and structure of Wnt Inhibitor IWP-3 were determined by mass spectrometry and NMR, respectively. Cellular toxicity of Wnt Inhibitor IWP-3 was tested on mouse ES cells.
References	Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., Lum, L. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-7.
Related Products	Stemolecule™ Wnt Inhibitor IWP-4 (Cat. # 04-0036) Stemolecule™ Wnt Inhibitor IWP-2 (Cat. # 04-0034)
Notice to Purchaser	Purchaser represents and warrants that it will use Stemolecule™ Wnt Inhibitor IWP-3 purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.