Spec Sheet

Stemolecule™ Pifithrin-α

Catalog #	Size	Price (USD)	Quantity
04-0038	5 mg	$99.00	Qty:

Stemolecule™ Pifithrin-α (PFT-α) is a small molecule inhibitor of p53 dependent apoptosis and transcriptional activation for cyclin G, p21/waf1, and mdm2 expression. The reduction of p53 activity has been shown to enhance reprogramming efficiency of human and mouse somatic cells.

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Stemolecule™ Pifithrin-α

Stemolecule™ Pifithrin-α (PFT-α) is a reversible inhibitor of p53 dependent apoptosis and transcriptional activation for cyclin G, p21/waf1, and mdm2 expression. The reduction of p53 activity has been shown to enhance reprogramming efficiency of human and mouse somatic cells. PFT-α enhances cell survival after treatment with UV irradiation or cytotoxic compounds. PFT-α protects wild-type mice against lethal doses of radiation without inducing visible tumors. The possibility exists that PFT-α may be used to reduce the side effects of radiation or chemotherapy for cancers that have lost p53 functions without affecting sensitivity of the tumors to treatment.

References

Komarov, P. G., Komarova, E. A., Kondratov, R. V., Christov-Tselkov, K. , Coon, J. S., Chernov, M. V. , Gudkov, A. V. (1999) A Chemical inhibitor of p53 that protects mice from the side effects of cancer therapy. Science 285: 1733- 1737.
Komarova, E. A., Gudkov, A. V. (2002) Suppression of p53: a new approach to overcome side effects of antitumor therapy. Biochemistry (Mosc) 65:41-48.
Kawamura, T., Suzuki, J. , Wang, Y., Menendez, S. Morera, L. Raya, A., Wahl, G., Belmonte, J. (2009) Linking the p53 tumour suppressor pathway to somatic cell reprogramming. Nature 460: 1140-1144.

For research use only. Not for use in diagnostic procedures. Not for resale.

Stemolecule™ Pifithrin-α

Size / Quantity	5 mg
Alternate Name	PFT-α, 2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolyethanone hydrobromide, 1-(4-Methylphenyl)-2-(4,5,6,7-tetrahydro-2-imino-3(2H)benzothiazoyl)ethanone HBr
CAS Number	63208-82-2
Chemical Formula	C₁₆H₁₈N₂OS-HBr
Structure
Formulation	Off-white solid
Molecular Weight	367.30
Purity	≥95%
Solubility	Soluble in DMSO (54 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 1361 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
Storage	Store at 4^oC. Protect from light. Following reconstitution, store aliquots at -20C.
Stability	Stock solutions stable at -20°C for 6 months.
Quality Control	The purity of Pifithrin-α was determined by HPLC analysis. The accurate mass of Pifithrin-α was determined by mass spectrometry. Cellular toxicity of Pifithrin-α was tested on mouse embryonic stem cells.
References	Komarov, P. G., Komarova, E. A., Kondratov, R. V., Christov-Tselkov, K. , Coon, J. S., Chernov, M. V. , Gudkov, A. V. (1999) A Chemical inhibitor of p53 that protects mice from the side effects of cancer therapy. Science 285: 1733- 1737. Komarova, E. A., Gudkov, A. V. (2002) Suppression of p53: a new approach to overcome side effects of antitumor therapy. Biochemistry (Mosc) 65:41-48. Kawamura, T., Suzuki, J. , Wang, Y., Menendez, S. Morera, L. Raya, A., Wahl, G., Belmonte, J. (2009) Linking the p53 tumour suppressor pathway to somatic cell reprogramming. Nature 460: 1140-1144.
Notice to Purchaser	Purchaser represents and warrants that it will use Stemolecule™ Pifithrin-α purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.