|
Product Overview
Stemolecule™ Cyclic Pifithrin-α (Cyclic PFT-α) is a transcriptional inhibitor of p53. Cyclic PFT-α was found to be one log more potent than the p53 inactivator Pifithrin-α with EC50 values ranging around 30 nM. The reduction of p53 activity has been shown to enhance reprogramming efficiency of human and mouse somatic cells. Cyclic PFT-α sensitizes tumor cells to antimicrotubule agent induced apoptosis.
References
- Pietrancosta, N., Maina, F., Dono, R., Moumen, A., Garino, C., Laras, Y., Burlet, S., Quelever, G., Kraus J. (2005) Novel cyclized Pifithrin-α inactivators: synthesis and biological studies. Bioorg Med Chem Lett 15: 1561-1564.
- Zuco, V., Zunino, F. (2005) Cyclic Pifithrin- α sensitizes wild type p53 tumor cells to antimicrotubule agent- induced apoptosis. Neoplasia 10: 587-596.
For research use only. Not for use in diagnostic procedures. Not for resale.
Product Specifications
| Size / Quantity |
5 mg
|
| Alternate Name |
Cyclic PFT-α hydrobromide, Pifithrin-α, cyclic, 2-(4-Methylphenyl)imidazo[2,1-b]-5,6,7,8-tetrahydrobenzothiazole hydrobromide
|
| Chemical Formula |
C16H18N2S-HBr
|
| Structure |
![[structure graphics]](http://www.stemgent.com/products/04-0040/images/structure.jpg)
|
| Formulation |
White to off-white solid
|
| Molecular Weight |
349.29
|
| Purity |
≥95%
|
| Solubility |
Soluble in DMSO (57 mM). For a 10 mM concentrated stock solution, reconstitute the compound by adding 1458 µl of DMSO to the entire contents of the vial. Note: for most cells, the maximum tolerance to DMSO is <0.5%.
|
| Storage |
Store at 4oC. Protect from light. Following reconstitution, store aliquots at -20C.
|
| Quality Control |
The purity of Cyclic Pifithrin-α was determined by HPLC analysis. The accurate mass of Cyclic Pifithrin-α was determined by mass spectrometry. Cellular toxicity of Cyclic Pifithrin-α was tested on mouse embryonic stem cells.
|
| Notice to Purchaser |
Purchaser represents and warrants that it will use Stemolecule™ Cyclic Pifithrin-α
purely for research purposes, not for diagnostic use, not
for resale, and not for use in humans or veterinary applications.
Stemgent will not be held responsible for patent infringement or
other violations that may occur with the use of our products.
|
| Stability |
Stock solutions stable at -20°C for 6 months.
|
Technical Documents
Specifications
MSDS
|
|
