Stemgent® Rat anti-Mouse OC2-4E8 Antibody

Catalog #
09-0063
Size
50 µg (100 µl at 0.5 mg/ml)
Price (USD)
$189.00
Qty
Order Offline
Tel: 877.228.9783
Email: orders@stemgent.com
Fax: 617-494-0398

Product Overview

The Stemgent® Rat anti-Mouse OC2-4E8 Antibody is a novel monoclonal antibody that reacts with a cell surface molecule on liver periductal cells. The OC2-4E8 clone was generated by immunization with nonparenchymal cells from 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) treated mice in order to increase representation of rare cell types such as oval cells. Oval cells are thought to be bipotential progenitors capable of yielding both hepatocytes and bile ducts. The OC2-4E8 antibody has been confirmed to stain periductal cells on mouse liver sections by immunohistochemistry and to stain viable dissociated liver cells by flow cytometry. The Stemgent® Rat anti-Mouse OC2-4E8 Antibody has been identified as a useful tool for tracing the development of specific liver cell progenitors in the study of mouse models of hepatic cell development1.

References

  1. Dorrell, C., Erker, L., Lanxon-Cookson, K., Abraham, S., Victoroff, T., Ro, S., Canaday, P., Streeter, P., Grompe, M. (2008) Surface Markers for the Murine Oval Cell Response. Hepatology 48(4):1282-1291.
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Immunofluorescent staining of 21-day DDC-treated mouse liver frozen section with Stemgent® Rat anti-Mouse OC2-4E8 Antibody. The OC2-4E8 antibody was detected using a Cy3-conjugated anti-rat IgG (red) and counterstained with Hoechst 3342 (blue)
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For research use only. Not for use in diagnostic procedures. Not for resale.

Product Specifications

Size / Quantity 50 µg (100 µl at 0.5 mg/ml)
Immunogen Nonparenchymal cells from DDC-treated mice
Reactivity Mouse
Clone OC2-4E8
Isotype Rat IgG1
Formulation Serum-free media with 0.09% sodium azide as preservative.
Storage Store at 4oC. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by immunohistochemistry (IHC, Figure 1). For a general IHC protocol, visit Stemgent® Rat anti-Mouse OC2-4E8 Antibody.
Recommended Usage Dilute 1:100 for immunohistochemistry applications. For use on tissue sections, acetone-fixed frozen sections are recommended. Paraformaldehyde or formalin fixation is not recommended. For flow cytometric analysis investigators should titrate the antibody to determine optimal concentration for usage.
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Notice to Purchaser Purchaser represents and warrants that it will use Stemgent® Rat anti-Mouse OC2-4E8 Antibody purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.

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