Characterization is a critical step in confirming status of pluripotent stem cell (iPS cell) cultures during reprogramming and at all stages of differentiation. Since this is a crucial, yet routine process, confirmation of cell status should be simple, quick and above all reliable. Stemgent offers a wide range of antibody formats and alkaline phosphatase kits that were developed and tested directly on ES/iPS cells or differentiated somatic cells.

The Stemgent™ StainAlive™ antibody format allows for direct staining of live cells in culture and therefore eliminates the need to sacrifice valuable material that can be used for downstream experiments.


Stemgent® StainAlive™ Antibodies



Human H1 ES cells were analyzed live in culture with Stemgent StainAlive DyLight™ 488 TRA-1-60 antibody. Light field microscopy was used to visualize colonies. Direct ICC analysis of live cells in culture  allows visualization of pluripotent cells without having to sacrifice valuable wells. 


Stemgent Alkaline Phosphatase (AP) Staining Kit II

The state of differentiation can be monitored by detection of AP, along with stem cell markers such as transcription factors Oct-3/4, Nanog, Sox-2 and surface markers SSEA-1, -3, -4, TRA-1-60 and TRA-1-81. Although stem cells from different sources or species display different cell surface markers, AP is a universal marker for all types of pluripotent stem cells. The Stemgent® (AP) Staining Kit II uses an easy, histochemical staining protocol with substrate that is stable for use up to 30 minutes allowing for greater throughput.

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Products (19)
Brand Name Catalog Number Size Price Quantity
Stemgent® Alkaline Phosphatase Staining Kit
00-0009 50 assays Ask For Price
Stemgent® Alkaline Phosphatase Staining Kit II
00-0055 50 assays $97.00
ReproCELL Rabbit anti-Human Nanog Polyclonal Antibody
RCAB003P 200 uL $500.00
RCAB004P-F 100 uL $300.00
ReproCELL Rabbit anti-Mouse Nanog Polyclonal Antibody
RCAB001P 200 uL $500.00
RCAB002P-F 100 uL $300.00
StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse anti-Mouse/Human
09-0095 100 μl $279.00
StainAlive™ SSEA-4 Antibody (DyLight™ 550), Mouse anti-Human
09-0097 100 μl $279.00
Stemgent® Stemgent® Goat anti-Rabbit IgG Antibody (DyLight™ 488)
09-0034 200 μl $89.00
Stemgent® Stemgent® Klf4 Antibody (Purified), Mouse anti-Mouse/Human
09-0021 100 μl $153.00
Stemgent® Stemgent® Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human
09-0020 100 μl $285.00
Stemgent® Stemgent® Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
09-0023 100 μl $197.00
Stemgent® Stemgent® Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
09-0024 100 μl $182.00
Stemgent® Stemgent® SSEA-3 Antibody (Purified), Rat anti-Mouse/Human
09-0014 100 μl $153.00
Stemgent® Stemgent® SSEA-4 Antibody (Purified), Mouse anti-Human
09-0006 100 μl $186.00
StainAlive™ Stemgent® StainAlive™ Mouse IgM, κ Isotype Control (DyLight™ 488)
09-0072 100 μl $110.00
StainAlive™ Stemgent® StainAlive™ SSEA-1 Antibody (DyLight™ 488), Mouse anti-Mouse/Human
09-0067 100 μl $279.00
StainAlive™ Stemgent® StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human
09-0068 100 μl $399.00
StainAlive™ Stemgent® StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human
09-0069 100 μl $399.00
Stemgent® Stemgent® TRA-1-60 Antibody (Purified), Mouse anti-Human
09-0010 100 μl $216.00
Stemgent® Stemgent® TRA-1-81 Antibody (Purified), Mouse anti-Human
09-0011 100 μl $109.00