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Reprogramming

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Reprogramming the industry
Solutions from Stemgent

Since the pioneering work by Dr. Shinya Yamanaka describing the reprogramming of somatic cells to a pluripotent-like state, the induced pluripotent stem (iPS) cell has become an invaluable tool for strategies in regenerative medicine, disease modeling, drug discovery, and basic research in cell development.

From traditional reprogramming methods (lentivirus, adenovirus) to non-integrating, non-viral technologies (mRNA, proteins), Stemgent provides the quality, knowledge, and expertise you depend on.

 

Choosing a reprogramming system

Stemgent offers a broad portfolio of cellular reprogramming systems to choose from - learn which system may be right for you.

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Even faster reprogramming

Generate virus-free iPS cell colonies in as early as 16 days. System saves time from needing to screen cells post-transfection.

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Compare reprogramming systems
See which system is right for your research

  mRNA Proteins Adenovirus Retrovirus Lentivirus
Product description Fastest, safest technology for non-integrating, virus-free generation of iPS cells Non-integrating, non-viral approach to reprogramming. Low-efficiency. Transient virus transduction method, DNA transgenes do not need to integrate for expression RNA virus that is duplicated in a host cell using the reverse transcriptase enzyme to produce DNA from its RNA genome. Low efficiency. Highly referenced technology for iPS cell generation using viral systems
Reprogramming efficiency >1% 0.00001% 0.0001 - 0.001% 0.001 - 0.01% 0.001 - 0.01%
Virus-free check check      
No screening required* check check      
Genomic integration-free check check      
Time to generate usable iPS cell lines >3 weeks       ~9 weeks
Validated protocol (s) check       check
Clinically-relevant check        
Bio-safety requirements None None BL2 BL2 BL2/BL2+
Recommended use Potential clinical-use, scalability, control protein expression Studying basic mechanisms of reprogramming For transient virus system applications   Designed to study the mechanisms of reprogramming
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Benefits of using the Stemgent mRNA Reprogramming System as compared to other reprogramming methods

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mRNA provides efficiencies greater than 1% as compared to other methods, which yield reprogramming efficiencies varying from 0.00001-0.01%. mRNA reprogramming does not require multi-step passaging or screening for virus or genomic integration once new colonies are derived.


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Experimental timeline comparison
Generate colonies in as early as 16 days with mRNA reprogramming

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Experiment timeline comparison shows mRNA reprogramming exhibits emerging virus-free iPS cells in less than 2 weeks (as compared to other systems that require additional work to screen cells for the presence of viral remnants).

Total time to generate a characterized iPS cell line using virus-based systems can take up to 25 weeks, whereas the Stemgent mRNA Reprogramming System can take as little as 9 weeks to generate a fully characterized and banked iPS cell line.

Note: Timelines are provided as a guideline for experimental planning and actual timelines can vary based on the cell type and experimental conditions.