RNA Reprogramming Technology
Learn the fundamentals of reprogramming from the leaders in the field.
Reprogramming Training Courses by Stemgent, a ReproCELL Group Company cover all of the essential protocols for microRNA-enhanced mRNA reprogramming and human induced pluripotent (iPS) cell culture techniques in a hands-on laboratory environment. These courses are designed to provide personalized instruction and to enable researchers to easily transfer essential techniques to reprogramming experiments carried out in their own labs.
Stemgent offers a three-day training course on microRNA-enhanced mRNA reprogramming and the fundamentals of human iPS cell culture, characterization and maintenance. All techniques and materials used in the RNA Reprogramming Courses are accompanied with detailed protocols, including a comprehensive reference manual. The methods and protocols for the ReproCELL Stemgent RNA reprogramming system have been optimized and fully validated on normal and disease-specific patient cells, for the efficient and reproducible generation of stable and fully pluripotent iPS cell lines.
Stemgent’s experienced and knowledgeable course instructors have been trained in the laboratories of leading stem cell scientists, including: Prof. Peter Andrews, and Dr. Rudolf Jaenisch. We are committed to providing follow-up technical support to all participants as they reprogram in their own labs following the course.
Host RNA Reprogramming Training on Your Campus!
Would your group benefit from personalized instruction at your own facility? We can bring the technology to you! ReproCELL's Stemgent technical conducts customized on-site trainings and workshops throughout the year. Learn how Stemgent can accelerate your team’s reprogramming efforts and boost your reprogramming expertise by working side-by-side with our scientists to train your own research staff. Depending on your need, in-house training opportunities can range from short seminars, interactive workshops, to full training courses.
To inquire about in-house training opportunities, please e-mail firstname.lastname@example.org.
Reprogramming somatic cells with the mRNA/microRNA enhanced reprogramming system enables the fast, safe, and efficient generation of clinically-relevant human iPS cell lines from difficult to reprogram cells. RNA-based reprogramming eliminates the risk of genomic integration and mutagenesis inherent to DNA and viral-based technologies. With the safest and fastest technology available, cells can be reproducibly reprogrammed with mRNA/microRNA in as little as 14 days and expanded as stable iPS cell lines without the need for subcloning or multi-step screening.
RNA Reprogramming 101 Course
Stemgent’s mRNA Reprogramming 101 Course is a comprehensive three-day training course, specifically focusing on the practical use of the mRNA/microRNA reprogramming system to generate human iPS cells. In addition to reprogramming techniques, the course will also cover all of the essential protocols for human iPS cell culture, maintenance and characterization. The RNA Reprogramming Course is designed to enable researchers to easily transfer essential techniques to successful mRNA/microRNA enhanced reprogramming experiments carried out in their own labs. This is a complete training course, perfect for researchers wanting to gain experience in human iPS cell culture, entering the field of reprogramming or interested in the latest technology and advancements in reprogramming somatic cells with mRNA/microRNA.
- Fundamentals of reprogramming using mRNA/microRNA
- Detailed overview of mRNA transfection and reprogramming protocols
- Reprogramming patient samples and understanding variation in target cell types
- Changes in cell morphology and the onset of pluripotency during reprogramming
- iPS cell characterization and proofs of pluripotency
- Human iPS cell culture techniques
- Experimental optimization and troubleshooting
Practical Lab Activities
- Preparation of mRNA/microRNA transfection complex and transfection of target cells
- Evaluating transfection efficiency using nGFP
- Observation of emerging morphology changes throughout the reprogramming timeline
- Mechanical colony picking and replating
- Characterization of live iPS cell colonies in culture with StainAlive™ antibodies
- iPS cell line maintenance and passaging
- Cryopreservation and thawing of human iPS cells
- Embryoid body (EB) formation and culture
Who Should Attend
- All researchers interested in learning about the specifics of reprogramming human cells
- Researchers interested in reprogramming cells with a safe, non-integrative, non-viral, and xeno-free system to generate clinically-relevant human pluripotent stem cell lines
- Researchers entering the field or those who are new to human iPS cell culture
- All researchers who are preparing or designing their RNA reprogramming experiments
Upcoming RNA Reprogramming 101 Courses
|May 6-8, 2015||Sheffield, UK||View Course Flyer||Register|
|May 11-13, 2015||Sheffield, UK||View Course Flyer||Register|
The course tuition includes lunches and refreshments each day of the course, as well as a detailed RNA reprogramming course manual. Travel and accommodations are not included in this fee.
For Stemgent courses held at the Centre for Stem Cell Biology (CSCB) at the University of Sheffield, please register directly through the CSCB at the information below. Course registrants will be contacted with confirmation of course reservation and directed to a payment site at the University of Sheffield to complete enrollment.
To ensure plenty of opportunities for one-on-one interaction and discussion, course sizes are limited to 12 participants per course. Reservations are given under a first come, first serve policy.
For more information and to register, visit the CSCB website at:
For more information about these courses or other training opportunities, please contact:
Meet the Trainers
Brad Hamilton, Director of Research & Development, Stemgent a ReproCELL Group Company
Brad Hamilton, is the Director of Research and Development for Stemgent’s stem cell focused programs, including the coordination of external academic and commercial collaborations and technology evaluations.
Brad received his B.S. in Biology from James Madison University and his M.S. in Biotechnology from the University of Tennessee-Knoxville and has eighteen years of research experience in both industry and academic labs. During that time, Mr. Hamilton has worked at Upstate Biotechnology and Millipore, leading R&D groups in the development of RNAi and cell signaling reagents. In addition, his time spent in academic research has focused on assay development for neurodegenerative disorders.
Christian Unger, PhD, University of Sheffield, Postdoctoral Research Associate in the lab of Peter Andrews
Christian Unger is an engineer and stem cell scientist with more than 10 years experience in working with human pluripotent stem cells. He started his University education in engineering and finished in 2002 with a degree in Biotechnology from the University of Applied Sciences in Jena, Germany. For his doctoral research he was part of a newly formed human embryonic stem cell network at the Karolinska Institutet in Stockholm, Sweden. Within the cell and gene therapy group of Dr. Siraç Dilber Christian studied and developed the culture of human embryonic stem cells towards xeno-free conditions.
Dr. Unger then joined the laboratory of Dr. Andrews in 2008 to establish a lentiviral facility for the generation of iPS cells. The advent of mRNA reprogramming in 2010 and his collaboration with Stemgent Inc. helped to establish integration-free reprogramming methods for the development of high-quality iPS cells. His iPS cell work and the expertise of Dr. Andrews laboratory led to the exploration of iPS cells as a disease model and in particular for the study of cancer. Dr. Unger is currently using iPS cells to study early initiation of embryonal cancers.