Antibodies

Characterization is a critical step in confirming status of pluripotent stem cell (iPS cell) cultures during reprogramming and at all stages of differentiation. Since this is a crucial, yet routine process, confirmation of cell status should be simple, quick and above all reliable. Stemgent offers a wide range of antibody formats and alkaline phosphatase kits that were developed and tested directly on ES/iPS cells or differentiated somatic cells.

The Stemgent™ StainAlive™ antibody format allows for direct staining of live cells in culture and therefore eliminates the need to sacrifice valuable material that can be used for downstream experiments.

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Stemgent® StainAlive™ Antibodies

 

 

Human H1 ES cells were analyzed live in culture with Stemgent StainAlive DyLight™ 488 TRA-1-60 antibody. Light field microscopy was used to visualize colonies. Direct ICC analysis of live cells in culture  allows visualization of pluripotent cells without having to sacrifice valuable wells. 

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Stemgent Alkaline Phosphatase (AP) Staining Kit II

The state of differentiation can be monitored by detection of AP, along with stem cell markers such as transcription factors Oct-3/4, Nanog, Sox-2 and surface markers SSEA-1, -3, -4, TRA-1-60 and TRA-1-81. Although stem cells from different sources or species display different cell surface markers, AP is a universal marker for all types of pluripotent stem cells. The Stemgent® (AP) Staining Kit II uses an easy, histochemical staining protocol with substrate that is stable for use up to 30 minutes allowing for greater throughput.

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Products (24)
Brand Name Catalog Number Size Price Quantity
Stemgent® Alkaline Phosphatase Staining Kit
00-0009 50 assays $0.00
Stemgent® Alkaline Phosphatase Staining Kit II
00-0055 50 assays $97.00
ReproCELL Rabbit anti-Human Nanog Polyclonal Antibody
RCAB003P 200 uL $500.00
RCAB004P-F 100 uL $300.00
ReproCELL Rabbit anti-Mouse Nanog Polyclonal Antibody
RCAB001P 200 uL $500.00
RCAB002P-F 100 uL $300.00
StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse anti-Mouse/Human
09-0095 100 μl $279.00
StainAlive™ SSEA-4 Antibody (DyLight™ 550), Mouse anti-Human
09-0097 100 μl $279.00
Stemgent® Stemgent® Goat anti-Rabbit IgG Antibody (DyLight™ 488)
09-0034 200 μl $89.00
Stemgent® Stemgent® Klf4 Antibody (Purified), Mouse anti-Mouse/Human
09-0021 100 μl $153.00
Stemgent® Stemgent® Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human
09-0020 100 μl $285.00
Stemgent® Stemgent® Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
09-0023 100 μl $197.00
Stemgent® Stemgent® Pax6 Antibody (Purified), Rabbit anti-Mouse/Human
09-0075 100 μl $285.00
Stemgent® Stemgent® Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
09-0024 100 μl $182.00
Stemgent® Stemgent® SSEA-1 Antibody (PE), Mouse anti-Mouse/Human
09-0001 1 ml $175.00
Stemgent® Stemgent® SSEA-1 Antibody (Purified), Mouse anti-Mouse/Human
09-0005 100 μl $197.00
Stemgent® Stemgent® SSEA-3 Antibody (PE), Rat anti-Human
09-0044 1 ml $164.00
Stemgent® Stemgent® SSEA-3 Antibody (Purified), Rat anti-Mouse/Human
09-0014 100 μl $153.00
Stemgent® Stemgent® SSEA-4 Antibody (PE), Mouse anti-Human
09-0003 1 ml $175.00
Stemgent® Stemgent® SSEA-4 Antibody (Purified), Mouse anti-Human
09-0006 100 μl $186.00
StainAlive™ Stemgent® StainAlive™ Mouse IgM, κ Isotype Control (DyLight™ 488)
09-0072 100 μl $110.00
StainAlive™ Stemgent® StainAlive™ SSEA-1 Antibody (DyLight™ 488), Mouse anti-Mouse/Human
09-0067 100 μl $279.00