RNA Reprogramming Systems for Fibroblasts and Blood
Highly Efficient Reprogramming
The Stemgent RNA Reprogramming System provides efficiencies greater than 1% as compared to other methods, which yield reprogramming efficiencies varying from 0.00001% to 0.1%. In addition to increased yield of colonies and fast reprogramming kinetics, RNA-based reprogramming does not require laborious multi-step downstream passaging or screening for viral vector retention or genomic integration.
The Stemgent microRNA-boosted mRNA reprogramming system, combining the mRNA Reprogramming Kit (00-0071) with the microRNA Booster Kit (00-0073) and the Stemfect™ RNA Transfection Kit (00-0069), provides high-quality reprogramming of adult and neonatal fibroblasts. The microRNA Booster Kit (00-0073) increases the success with highly refractory target cells, improves efficiency, and reduces the cost per well.
The Stemgent StemRNA™-SR Reprogramming Kit (00-0075), using self-replicative RNA, is the first RNA-based reprogramming kit for blood derived cells. Starting with genetically stable endothelial progenitor cells (EPCs), easily isolated from whole blood or frozen MNCs, this technology can efficiently generate iPSCs that are free integrated vector and ready to differentiate or scale up.