Stemfactor™ Activin A, Human Recombinant

Catalog Size Price Quantity
03-0001 5 μg $158.00
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Product Overview

Stemfactor Activin A is a crystallography-grade preparation providing the highest purity available. Activin A is bi-modal in action having been shown to maintain pluripotency of stem cells and promote differentiation1,2,3,4. Mature recombinant human Activin A is a ~25 kDa disulfide-linked homodimer of two 116 amino acid residue βA subunits.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplment necessary to support naive pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor™ LIF, Human Recombinant (03-0016), and

A: Stemfactor™ Activin A, Human Recombinant (03-0001)


Product Specifications

Size

5 μg

Source

E.Coli

Formulation

Lyophilized from 20% acetonitrile

Amino Acid Sequence

GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Uniprot Accession Number

P08476, residues 311-426

Purity

Greater than 98% by SDS-PAGE analysis

Endotoxin Level

Less than 1.0 EU/μg of Activin A as determined by the LAL method.

Biologic Activity

The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

Storage and Stability

Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at -20°C to -80°C.  Reconstituted Activin A, at concentrations greater than 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and for 1 year when stored at -80°C

 Reconstitution

Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.


Specification Sheets

Safety Data Sheets

References

  1. Beattie, G.M., Lopez, A.D., Bucay, N., Hinton, A., Firpo, M.T., King, C.C., and Hayek, A. (2005) Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23: 489-495.
  2. Vallier, L., Alexander, M., and Pedersen, R.A. (2005) Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J. Cell Sci. 118: 4495-4509.
  3. Sulzbacher, S., Schroeder, I.S., Truong, T.T., and Wobus, A.M. (2009) Activin A-induced differentiation of embryonic stem cells into endoderm and pancreatic progenitors- the influence of differentiation factors and culture conditions. Stem Cell Rev. 5: 159-173.
  4. Valdimarsdottir, G., and Mummery, C. (2005) Functions of the TGFbeta superfamily in human embryonic cells. APMIS 113: 773-789.

Additional Publications



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