Stemolecule™ Wnt Inhibitor IWP-3

Catalog Size Price Quantity
04-0035 2 mg $99.00
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04 0035 Figure 1

Product Overview

Stemolecule Wnt Inhibitor IWP-3 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1. The inhibition of the Wnt pathway through the use of IWP-3 has also been shown to promote the formation of cardiocytes from human embryonic stem cell-derived mesoderm cells2


Product Specifications

Size

2 mg

Alternate Name

2-(3-(4-fluorophenyl)-3,4,6,7-tetrahydro-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide

Chemical Formula

C22H17FN4O2S3

Molecular Weight

484.59

CAS Number

687561-60-0

Purity

Greater than 99% by HPLC analysis 

Formulation

Light yellow solid

Solubility

For a 10 mM concentrated stock solution of Wnt Inhibitor IWP-3, reconstitute the compound by adding 412.7 μl of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.

Storage and Stability

Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality control

The purity of Wnt Inhibitor IWP-3 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry.  Cellular toxicity of Wnt Inhibitor IWP-3 was tested on mouse embryonic stem cells. 

Related Products

  • Stemolecule™ Wnt Inhibitor IWP-4 (Cat.No.04-0036)
  • Stemolecule™ Wnt Inhibitor IWP-2 (Cat.No.04-0034)

Specification Sheets

Safety Data Sheets



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