Stemolecule™ Wnt Inhibitor IWP-4

Catalog Size Price Quantity
04-0036 2 mg $99.00
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04 0036 Figure 1

Product Overview

Wnt Inhibitor IWP-4 was identified in a high throughput screen for antagonists of the Wnt / β-catenin pathway. Wnt Inhibitor IWP-4 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, there by blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1.  


Product Specifications

Size

2 mg

Chemical Name

2-(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide

Chemical Formula

C23H20N4O3S3

Molecular Weight

496.62

CAS Number

686772-17-8

Purity

Greater than 96% by HPLC analysis 

Formulation

White solid

Solubility

Wnt Inhibitor IWP-4 molecule is soluble in DMSO at 1.2 mM.

Reconstitution

For a 1.2 mM concentrated stock solution of Wnt Inhibitor IWP-4, reconstitute the compound by adding 3.36 ml of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For use in cell culture, warm the medium just prior to adding the reconstituted compound. Once the compound is added, mix and filter-sterilize the medium using a 0.2 µM low-protein binding filter.  

Storage and Stability

Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality control

The purity of Wnt Inhibitor IWP-4 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM - 25 µM of Wnt Inhibitor IWP-4 .


Specification Sheets

Safety Data Sheets

References

  1. Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-107.

Additional Publications



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