StainAlive™ Stemgent® StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human

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09-0068 100 μl $399.00
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09-0068_TRA-1-60_Immunocytochemical_H1humanES
Immunocytochemical analysis of TRA-1-60 on H1 human ES cells. Phase contrast view of cells in culture and the same field of view after staining with StainAlive TRA-1-60 Antibody (DyLight 488) using a 1:100 dilution.

Product Overview

The TRA-1-60 antibody reacts with a pluripotent, stem cell specific antigen expressed on undifferentiated human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

This antibody is ideal for live-staining cells, because it has been specifically formulated with low endotoxin and no sodium azide. This allows in-process monitoring of reprogramming experiments without sacrificing cells. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with StainAlive TRA-1-60 antibody. 


Product Specifications

Size

100 μl

Concentration

0.5 mg/ml

Clone

TRA-1-60

Isotype

Mouse IgM, κ

Immunogen

Human embryonal carcinoma (EC) cell line 2102Ep

Reactivity

Human

Preparation

This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 μm filter sterilized. Formulation is free from bacteria, fungi, mycoplasma, and mouse virus.

Formulation

Phosphate-buffered solution, pH 7.2, containing no preservative

Storage and Stability

Store at 4°C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry to ensure product quality.
Endotoxin level is less than 0.1 EU/μg (0.01 ng/μg) of the protein as determined by the LAL assay.

Recommended Usage

The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please reference Protocol: Immunocytochemistry of Live Cells online at www.stemgent.com/support/protocols.


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References

  1. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.
  3. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.
  4. Chin, A.C., Fong, W.J., Goh, L.T., Philp, R., Oh, S.K., and Choo, A.B. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 130: 320-328.
  5. Badcock, G., Pigott, C., Goepel, J., and Andrews, P.W. (1999) The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 59: 4715-4719.
  6. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.

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