StainAlive™ Stemgent® StainAlive™ Mouse IgM, κ Isotype Control (DyLight™ 488)

Catalog Size Price Quantity
09-0072 100 μl $110.00
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Product Overview

StainAlive Mouse IgM, κ Isotype Control (DyLight 488) is used as a negative control antibody for staining embryonic stem (ES) and induced pluripotent stem (iPS) cells in live culture. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and the expression of pluripotency markers does not change after staining with this antibody.


Product Specifications

Size

100 μl

Concentration

0.5 mg/ml

Clone

MM-30

Isotype

Mouse IgM, κ

Immunogen

Trinitrophenol + KLH

Preparation

This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 μm filter sterilized. Formulation is free from bacteria, fungi, mycoplasma, and mouse virus.

Formulation

Phosphate-buffered solution, pH 7.2, containing no preservative

Storage and Stability

Store at 4°C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.

Quality Control

Tested by immunocytochemistry to ensure product quality.
Endotoxin level is less than 0.1 EU/μg (0.01 ng/μg) of the protein as determined by the LAL assay.  

Recommended Usage

This antibody is used as an isotype control in immunofluorescent staining. Use at concentrations comparable to those of the specific antibody of interest. For an application specific protocol, please referenceProtocol: Immunocytochemistry of Live Cells online at www.stemgent.com/support/protocols.




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