Stemolecule™ SC1 (Pluripotin)

Catalog Size Price Quantity
04-0011 1 mg $99.00
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04 0011 Figure 1
The use of SC1 (Pluripotin) to support mESC self-renewal in the absence of LIF.
04 0011 Figure 2

Product Overview

SC1 (Pluripotin) was identified in a cell-based chemical  library screen for small   molecules that regulate mouse embryonic stem (ES) cell self-renewal. SC1 is sufficient to sustain long-term self-renewal of mouse ES cells in an undifferentiated state in the absence of LIF, feeder cells, or serum. SC1 acts through dual inhibition of extracellular signal-regulated kinase 1 (ERK1) and Ras GTPase-activating protein (Ras-GAP). Mouse ES cells maintained in the presence of SC1 retain their ability to differentiate into cells representing all three germ layers in vitro, form chimeric mouse, and contribute to germ line in vivo1


Product Specifications

Size

1 mg

Alternate Name

N-[3-[7-[(2,5-dimethylpyrazol-3-yl)amino]-1-methyl-2-oxo-4H-pyrimido[4,5-d]pyrimidin-3-yl]-4-methylphenyl]-3-(trifluoromethyl)benzamide

Chemical Formula

C27H25F3N8O2

Molecular Weight

550.54

CAS Number

839707-37-8

Purity

Greater than 98% by HPLC analysis 

Formulation

White solid

Solubility

For a 10 mM concentrated stock solution of SC1, reconstitute the compound by adding 181.6 μl of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100mM.

Storage and Stability

Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality Control

The purity of SC1 (Pluripotin) was determined by HPLC analysis. The accurate mass was determined by mass spectrometry.  Cellular toxicity of SC1 (Pluripotin) was tested on mouse embryonic stem cells. 


Specification Sheets

Safety Data Sheets



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