Stemfactor™ LIF, Mouse Recombinant

Catalog Size Price Quantity
03-0011 1 ml, 10 μg/ml $215.00
03-0011-100 1 ml, 100 μg/ml $859.00
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Product Overview

Mouse LIF is a lymphoid factor that promotes long-term maintenance of the pluripotency of mouse embryonic stem cells by suppressing spontaneous differentiation1,2. Leukemia Inhibitory Factor (LIF) has additional functions such as cholinergic neuron differentiation, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is an approximate 20 kDa protein containing 202 amino acid residues. 


Product Specifications

Size

1 ml

Concentration

10 μg/ml (Cat. No. 03-0011)
100 μg/ml (Cat. No. 03-0011-100)

Source

E. coli

Purity

Greater than 98% by SDS-PAGE analysis.

Formulation

LIF is supplied as a 0.22 µm sterile filtered liquid in PBS containing 1% w/v BSA as carrier.

Endotoxin Level

Less than 1 EU/μg of LIF as determined by the LAL method.

Biologic Activity

Mouse LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1 x 106 units/ml (Cat. No. 03-0011) and 1 x 107 units/ml (Cat. No. 03-0011-100), where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 ml of medium.

Recommended Usage

1 ml of mouse LIF with the specific activity of 1 x 106 units/ml is sufficient to treat 1 L of embryonic stem cell culture media.
1 ml of mouse LIF with the specific activity of 1 x 107 units/ml is sufficient to treat 10 L of embryonic stem cell culture media.

Sterility

Tested to be negative for mycoplasma by PCR and microbial contamination by a sterility test.

Storage and Stability

LIF is shipped at room temperature. Concentrated LIF is stable for up to 6 months from date of receipt when stored at 4°C. Prior to use dilute LIF in sterile tissue culture media, aliquot to a convenient concentration, and store at 4°C. Avoid freeze-thaw cycles as it can result in loss of activity.




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