Stemolecule™ RG108

Catalog Size Price Quantity
04-0001 10 mg $135.00
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04-0001 RG108 Figure 1

Product Overview

Stemolecule RG108 is a cell-permeablesmall molecule that inhibits DNA methyltransferases. Treatment with RG108 results in the demethylation of genomic DNA and can reactivate epigenetically silenced tumor suppressor genes, making it a great tool for studying methlytransferase biological functions in vivo andin vitro1,2. It is particularly useful due to its non-covalent binding to the enzyme activation domain, which makes the inhibition reversible. Along with BIX01294, RG108 has been shown to improve the reprogramming efficiency of mouse embryonic fibroblasts (MEFs) into induced pluripotent stem (iPS) cells3.  RG108 can potentially be used to maintain embryonic stem (ES) cells in an undifferentiated state as well as replace transcription factors in both mouse and human cell reprogramming.

Product Specifications


10 mg

Alternate Name

2-(1,3-Dioxo-1,3-dihydro- 2H-isoindol-2-yl)- 3-(1H-indol-3-yl)propionic acid

Chemical Formula


Molecular Weight


CAS Number



Greater than 99% by HPLC analysis


Yellow solid


For a 10mM concentrated stock solution of RG108, reconstitute the compound by adding 2.99ml of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100mM and ethanol at 100 mM. 

Storage and Stability

Store lypholized powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality Control

The purity of RG108 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of RG108 was tested on mouse embryonic stem cells. 

Specification Sheets

Safety Data Sheets


  1. Brueckner, B., Boy, R., Siedlecki, P., Musch, T., Kliem, H., Zielenkiewicz, P., Suhai, S., Wiessler, M., and Lyko, F. (2005) Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases. Cancer Res
  2. Stresemann, C., Brueckner, B., Musch, T., Stopper, H., and Lyko, F. (2006) Functional diversity of DNA methyltransferase inhibitors in human cancer cell lines. Cancer Res 66: 2794-2800.
  3. Shi, Y., Desponts, C., Do, J.T., Hahm, H.S., Schöler, H.R., and Ding, S. (2008) Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3: 568-574.

Additional Publications

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