Oct4 mRNA, Human

Catalog Size Price Quantity
05-0014 20 μg $250.00
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Figure 1: Protein expression and localization shown by immunocytochemistry, DAPI in blue and Oct4 in red.

Product Overview

Stemgent Oct4 mRNA encodes the Oct4 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2 Repeated transfection of Oct4 mRNA in combination with mRNAs encoding Klf4, Sox2, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.2

Product Specifications

Size

20 μg

Formulation

100 ng/μl in RNase-free TE buffer, pH 7.0

Storage and Stability

Store at or below -70°C. Stable for a minimum of 3 months from date of receipt when stored as directed. Wear gloves when handling this product. Use RNase-free tubes and barrier pipette tips. Thaw on ice. Keep on ice until the sample is removed. Avoid multiple freezing and thawing cycles (<5). Aliquot to experimental quantities.

Quality Control

Proper protein expression and localization have been confirmed by immunocytochemistry.

Recommended Usage

Stemgent Oct4 mRNA, Human should be transfected using a protocol specifically optimized for the cell type used in the experiment.

Related Products 

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  • Stemgent Sox2 mRNA, Human (Cat.No.05-0016)
  • Stemgent c-Myc mRNA, Human (Cat.No.05-0018)
  • Stemgent Lin-28 mRNA, Human (Cat.No.05-0017)
  • Stemgent nGFP mRNA (Cat.No.05-0019)
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  • Stemfect™ RNA Transfection Kit (Cat.No.00-0069)
  • Stemfactor™ Fibroblast Growth Factor-basic (Human Recombinant) (Cat.No.03-0002)
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Notice to Purchaser

This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic and prognostic use, (iii) any use in delivery to and/or modification of cells that are intended for clinical, diagnostic or medicinal use, including without limitation, cell-based therapy, (iv)any commercial purposes, including without limitation the performance of contract research or provision of services to a third party and the manufacture of products for general sale. Any use of this product for any of the abovementioned purposes requires a license from the Massachusetts Institute of Technology.



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