Klf4 mRNA, Human

Catalog Size Price Quantity
05-0015 20 μg $250.00
Add To Cart
Order Offline
T: 877.228.9783
F: 617.812.5900
E: orders@stemgent.com
thumb
thumb
Figure 1: Protein expression and localization shown by immunocytochemistry, DAPI in blue and Klf4 in red.

Product Overview

Stemgent Klf4 mRNA encodes the Klf4 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2 Repeated transfection of Klf4 mRNA in combination with mRNAs encoding Oct4, Sox2, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.2


Product Specifications

Size

20 μg

Formulation

100 ng/μl in RNase-free TE buffer, pH 7.0

Storage and Stability

Store at or below -70°C. Stable for a minimum of 3 months from date of receipt when stored as directed. Wear gloves when handling this product. Use RNase-free tubes and barrier pipette tips. Thaw on ice. Keep on ice until the sample is removed. Avoid multiple freezing and thawing cycles (<5). Aliquot to experimental quantities.

Quality Control

Proper protein expression and localization have been confirmed by immunocytochemistry.

Recommended Usage

Stemgent Klf4 mRNA, Human should be transfected using a protocol specifically optimized for the cell type used in the experiment.

Related Products 

  • Stemgent Oct4 mRNA, human (Cat.No.05-0014 )
  • Stemgent Sox2 mRNA, Human (Cat.No.05-0016)
  • Stemgent Lin-28 mRNA, Human (Cat.No.05-0017)
  • Stemgent nGFP mRNA (Cat.No.05-0019)
  • Stemgent c-Myc mRNA, Human (Cat.No.05-0018)
  • Stemgent mRNA Reprogramming Factors Set: hOKSML (Cat.No.00-0067)
  • Stemfect™ RNA Transfection Kit (Cat.No.00-0069)
  • Stemgent BJ Human Fibroblasts (early passage, p6) (Cat.No.08-0027)
  • Stemolecule™ Y27632 (Cat.No.04-0012)
  • Stemfactor™ Fibroblast Growth Factor-basic (Human Recombinant) (Cat.No.03-0002)

Notice to Purchaser

This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic and prognostic use, (iii) any use in delivery to and/or modification of cells that are intended for clinical, diagnostic or medicinal use, including without limitation, cell-based therapy, (iv)any commercial purposes, including without limitation the performance of contract research or provision of services to a third party and the manufacture of products for general sale. Any use of this product for any of the abovementioned purposes requires a license from the Massachusetts Institute of Technology.




Stemgent Comments and Reviews (0)

No comments or reviews yet. Be the first to leave a review.

Add a Comment

You must log-in or register as a Stemgent member to submit a comment.


You are fully responsible for the content you post. We encourage collaboration and helpful reviews among scientists and will use this feature to conduct helpful reviews of our products. Content that includes profanity, personal attacks or antisocial behavior (such as "spamming" or "trolling"), or other inappropriate content or material will be removed. We reserve the right to block any user who violates our terms of use, including removing all content posted by that user.