Stemolecule™ A83-01

Catalog Size Price Quantity
04-0014 2 mg $119.00
04-0014-10 10 mg $399.00
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04-0014 Figure 1

Product Overview

A83-01 is a selective inhibitor of the transforming growth factor-beta (TGF-β) type I receptor ALK5, the Activin/Nodal receptor ALK4, and the nodal receptor ALK71. This molecule is more potent than SB431542 in its inhibition of ALK4, 5, and 7, and only weakly inhibits ALK1, 2, 3, and 6. A83-01 inhibits the TGF-β-induced epithelial-to-mesenchymal transition (EMT) via the inhibition of Smad2 phosphorylation2.


Product Specifications

Size

2 mg (Cat. No. 04-0014)
10 mg (Cat. No. 04-0014-10)

Chemical Name

3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide

Chemical Formula

C25H19N5S

Molecular Weight

421.52

CAS Number

909910-43-6

Purity

Greater than 98% by HPLC analysis 

Formulation

Pale yellow solid

Solubility

 A83-01 is soluble in DMSO at 50 mM. 

Reconstitution

Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.

Storage and Stability

Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed.

Quality Control

The purity of A83-01 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM - 100 µM of A83-01. 


References

  1. Tojo, M., Hamashima, Y., Hanyu, A., Kajimoto, T., Saitoh, M., Miyazono, K., Node, M., and Imamura, T. (2005) The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta. Cancer Sci. 96: 791-800.
  2. Li, W., Wei, W., Zhu, S., Zhu, J., Shi, Y., Lin, T., Hao, E., Hayek, A., Deng, H., and Ding, S. (2009) Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell. 4: 16-19.

Additional Publications



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