Stemolecule™ Y27632 in Solution

Catalog Size Price Quantity
04-0012-02 2 mg (10 mM) $139.00
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Product Overview

Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor1. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency2. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors3. Y27632 in combination with Pifithrin-µ significantly improves cell recovery after cryopreservation4.

Stemolecule Y27632 in Solution is a ready to use 10 mM stock solution for stem cell culture.


Product Specifications

Size

2 mg

Concentration

10 mM in DMSO

Alternate Name

(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride

Chemical Formula

C14H23Cl2N3O

Molecular Weight

320.3

CAS Number

146986-50-7

Purity

Greater than 97% by HPLC analysis

Formulation

10 mM solution of Y27632 in DMSO (2 mg in 624.4 μl)

Handling

Before opening, briefly centrifuge the vial to ensure full recovery of sample. Aliquotting the stock solution is recommended to avoid repetitive freeze-thaw cycles. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%.

Storage and Stability

Store solution at -20°C protected from light. Stable for 6 months from date of receipt when stored as directed.

Quality Control

The purity of Y27632 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Y27632 was tested on mouse embryonic stem cells.


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References

  1. Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.
  2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.
  3. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.
  4. Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.

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