microRNA Booster Kit

Catalog Size Price Quantity
00-0073 kit $695.00
Add To Cart
Order Offline
T: 877.228.9783
F: 617.812.5900
E: orders@stemgent.com

Custom reprogramming services available. Learn more.

Stemgent microRNA-enhanced mRNA
Figure 1: MicroRNA Enhanced mRNA reprogramming experimental timeline. The Stemgent microRNA enhanced mRNA reprogramming protocol has been optimized to ensure that human iPS cell colonies are ready to expand in 2 weeks.
Stemgent mRNA
Figure 2: mRNA reprogramming experimental timeline. The Stemgent RNA reprogramming protocol has been optimized to ensure that human iPS cell colonies are ready to expand in 3 weeks.
iPS Cells microRNA-enhanced mRNA Reprogramming-Day1-4x
Figure 3: Time-course image progression for the generation of human iPS cells using the Stemgent-Asterand microRNA Enhanced mRNA Reprogramming System. Phase-contrast images show the progression of morphology changes at Day 1.
iPS Cells microRNA-enhanced mRNA Reprogramming-Day3-10x
Figure 4: Time-course image progression for the generation of human iPS cells using the Stemgent-Asterand microRNA Enhanced mRNA Reprogramming System. Phase-contrast images show the progression of morphology changes at Day 3.
iPS Cells microRNA-enhanced mRNA Reprogramming-Day5-10x
Figure 5: Time-course image progression for the generation of human iPS cells using the Stemgent-Asterand microRNA Enhanced mRNA Reprogramming System. Phase-contrast images show the progression of morphology changes at Day 5.
iPS Cells microRNA-enhanced mRNA Reprogramming-Day7-10x
Figure 6: Time-course image progression for the generation of human iPS cells using the Stemgent-Asterand microRNA Enhanced mRNA Reprogramming System. Phase-contrast images show the progression of morphology changes at Day 7.
iPS Cells microRNA-enhanced mRNA Reprogramming-Day9-10x
Figure 7: Time-course image progression for the generation of human iPS cells using the Stemgent-Asterand microRNA Enhanced mRNA Reprogramming System. Phase-contrast images show the progression of morphology changes at Day 9.
iPS Cells microRNA-enhanced mRNA Reprogramming-Day12-4x
Figure 8: Time-course image progression for the generation of human iPS cells using the Stemgent-Asterand microRNA Enhanced mRNA Reprogramming System. Phase-contrast images show the progression of morphology changes at Day 12.
iPS Cells microRNA-enhanced mRNA Reprogramming-Day12-4x-stain
Figure 9: Time-course image progression for the generation of human iPS cells using the Stemgent microRNA Enhanced mRNA Reprogramming System. Resulting iPS cell colonies stain positively for TRA-1-81 pluripotency marker expression on Day 12.

Product Overview

The Stemgent microRNA Booster Kit enhances, and simplifies mRNA reprogramming for fast, efficient iPS cell line generation from both normal and patient specific cell lines.The microRNA Booster Kit includes the microRNA Reprogramming Cocktail and B18R Recombinant Protein.

The microRNA-enhanced mRNA Reprogramming method improves upon the first generation mRNA Reprogramming Kit, delivering faster reprogramming kinetics, a streamlined protocol and improved efficacy on hard-to-reprogram or refractory cell types. The microRNA-enhanced method combines Stemgent’s mRNA Reprogramming Kit with the microRNA Booster Kit and the Stemfect™ RNA Transfection Kit to generate expandable primary iPS cell colonies in as little as 8 days. This new method improves kinetics and reduces mRNA dose per transfection resulting in a per well cost reduction of approximately 35% to 45% versus feeder-based mRNA Reprogramming.

 

 

Advantages

  Stemgent
mRNA Reprogramming Method
Stemgent
Enhanced microRNA
mRNA Reprogramming Method
Reprogramming efficiency >1% >1%
Fast Reprogramming Kinetics Yes Yes
Somatic Cell → iPS Cell 3 weeks 2 weeks
Risk of insertional mutagenesis None None
Screening for Viral Clearance None None
Screening for DNA vector retention and/or integration None None
Bio-containment issues associated with viral applications None None
# of wells per kit
(6 well plate format)
4 9-10
(35%-45% per well cost reduction)
Reprogramming Substrate Fibroblast feeder layer Extracellular Matrix
Captures hard-to reprogram and refractory cell types Yes
Simple, user friendly protocol (overnight transfections) Yes

Product Specifications

Size

1 kit

Kit Contents

  • 2 vials - microRNA Reprogramming Cocktail (20 µM) (Cat. No. 05-0036), 35 µL
  • B18R Recombinant Protein, Carrier-Free (Cat. No. 03-0017), 50 µg

Storage and Stability

  1. Store both the microRNA Reprogramming Cocktail and the B18R protein at or below -70 °C.
  2. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.

Quality Control

The Stemgent microRNA Booster Kit is functionally validated for successful RNA-based reprogramming of human newborn foreskin fibroblasts. All components of the kit are sterile and have tested negative for mycoplasma.

Recommended Usage

  • Refer to the microRNA-Enhanced mRNA Reprogramming Protocol for directions on use of the Stemgent microRNA Booster Kit.
  • Refer to the protocol Generating NuFF-Conditioned Pluriton Medium for instructions on production of the required reprogramming medium.
  • Refer to the User Manual: Stemgent mRNA Reprogramming System for additional details on materials and handling.



Stemgent Comments and Reviews (0)

No comments or reviews yet. Be the first to leave a review.

Add a Comment

You must log-in or register as a Stemgent member to submit a comment.


You are fully responsible for the content you post. We encourage collaboration and helpful reviews among scientists and will use this feature to conduct helpful reviews of our products. Content that includes profanity, personal attacks or antisocial behavior (such as "spamming" or "trolling"), or other inappropriate content or material will be removed. We reserve the right to block any user who violates our terms of use, including removing all content posted by that user.