ReproCELL ReproFF2 Cell Culture Medium

Catalog Size Price Quantity
RCHEMD006 500 mL $266.00
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RCHEMD006bottle
RCHEMD006cell
RCHEMD006weekend

Product Overview

- Culture maintenance required only three days/week.

- Reduce the labor cost for the weekend.

- Maintains undifferentiated state and differentiation capability.

- No need of mechanical manipulation for passaging when used with Laminin-5 and Dissociation Solution..

ReproFF2 is a new feeder-free culture medium for human ES/iPS cells. Unlike conventional stem cell media that requires changing on a daily basis, ReproFF2 needs culture maintenance only three days a week. Change the medium on Monday and Wednesday, passage the cells on Friday. Consequently, the cost and amount of medium used are halved, feeder cells need not be prepared, and the culture need not be cared during the weekend.

ReproFF2 has been developed to reduce the steps in the workflow. Just warm up, add bFGF*, and ReproFF2 is ready to use.


Product Specifications

Size

500 ml medium

Storage and Stability

Store basal medium at -20°C. Medium is stable for 3 years when stored as directed.

Quality Control

• Human embryonic stem cells (ES) and induced pluripotent stem
cells (iPS) can be maintained only 3 days a week of cultivation
(e.g., Monday, Wednesday, and Friday).
• Suitable for feeder-free culture of human ES/iPS cells.
• Each lot is culture-tested with human iPS cells as described in
Takahashi K, et al., Cell 2007;131:861–72.
• Lot-to-lot control testing of other critical criteria, including
osmolality, pH, sterility, and mycoplasma is performed.
• Serum-free.
*Includes 2-Mercaptoethanol.

 Note to Purchaser

 This product is for research use only, not for therapeutic or diagnostic purposes. It is not allowed to sell this product to a third party or use it for commercial purposes without permission from ReproCELL.




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