Stemfactor™ FGF-basic, Human Recombinant
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Fibroblast Growth Factor-basic (bFGF), also known as FGF-2, is a heparin-binding member of the FGF superfamily of molecules. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. Additionally, bFGF is a critical component of embryonic stem cell culture medium, allowing cells to remain in an undifferentiated state in serum-free medium1,2. Stemfactor bFGF is an approximate 17 kDa protein consisting of 155 amino acid residues.
Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl.
Amino Acid Sequence
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Uniprot Accession Number
P09038, residues 1-155
Greater than 95% by SDS-PAGE analysis
Less than 1.0 EU/μg of bFGF as determined by the LAL method.
The ED50 is 0.1 to 1 ng/ml as determined by the dose dependent proliferation of NIH 3T3 cells.
Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.
Storage and Stability
Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at -20°C to -80°C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and up to 6 months when stored at -80°C.
Centrifuge briefly and then reconstitute bFGF in 500 μl of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/ml of bFGF. Avoid freeze-thaw cycles as it can result in loss of activity.
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