Stemfactor™ FGF-basic, Human Recombinant

Catalog Size Price Quantity
03-0002 50 μg $137.00
Add To Cart
Order Offline
T: 877.228.9783
F: 617.812.5900
E: orders@stemgent.com

Product Overview

Fibroblast Growth Factor-basic (bFGF), also known as FGF-2, is a heparin-binding member of the FGF superfamily of molecules. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. Additionally, bFGF is a critical component of embryonic stem cell culture medium, allowing cells to remain in an undifferentiated state in serum-free medium1,2. Stemfactor bFGF is an approximate 17 kDa protein consisting of 155 amino acid residues. 


Product Specifications

Size

50 μg

Source

E. coli

Formulation

Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl.

Amino Acid Sequence

MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

Uniprot Accession Number

P09038, residues 1-155

Purity

Greater than 95% by SDS-PAGE analysis

Endotoxin Level

Less than 1.0 EU/μg of bFGF as determined by the LAL method.

Biologic Activity

The ED50 is 0.1 to 1 ng/ml as determined by the dose dependent proliferation of NIH 3T3 cells.

Sterility

Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.

Storage and Stability

Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at -20°C to -80°C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and up to 6 months when stored at -80°C.

Reconstitution

Centrifuge briefly and then reconstitute bFGF in 500 μl of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/ml of bFGF. Avoid freeze-thaw cycles as it can result in loss of activity.


Related Products

References

  1. Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thompson, J.A. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227: 271-278.
  2. Xu, C., Rosler, E., Jiang, J., Lebkowski, J.S., Gold, J.D., O’Sullivan, C., Delavan-Boorsma, K., Mok, M., Bronstein, A., and Carpenter, M.K. (2005) Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23: 315-323.
  3. Vallier, L., Alexander, M., and Pedersen, R.A. (2005) Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J. Cell Sci. 118: 4495-4509.
  4. Wang, G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D., and Deng, H. (2005) Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330: 934-942.
  5. Greber, B., Lehrach, H., and Adjaye, J. (2007) Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal. Stem Cells 25: 455-464.

Additional Publications



Stemgent Comments and Reviews (0)

No comments or reviews yet. Be the first to leave a review.

Add a Comment

You must log-in or register as a Stemgent member to submit a comment.


You are fully responsible for the content you post. We encourage collaboration and helpful reviews among scientists and will use this feature to conduct helpful reviews of our products. Content that includes profanity, personal attacks or antisocial behavior (such as "spamming" or "trolling"), or other inappropriate content or material will be removed. We reserve the right to block any user who violates our terms of use, including removing all content posted by that user.