Stemfactor™ FGF-basic, Human Recombinant

Catalog Size Price Quantity
03-0002 50 μg $137.00
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T: 877.228.9783
F: 617.812.5900

Product Overview

Fibroblast Growth Factor-basic (bFGF), also known as FGF-2, is a heparin-binding member of the FGF superfamily of molecules. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. Additionally, bFGF is a critical component of embryonic stem cell culture medium, allowing cells to remain in an undifferentiated state in serum-free medium1,2. Stemfactor bFGF is an approximate 17 kDa protein consisting of 155 amino acid residues. 

Product Specifications


50 μg


E. coli


Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl.

Amino Acid Sequence


Uniprot Accession Number

P09038, residues 1-155


Greater than 95% by SDS-PAGE analysis

Endotoxin Level

Less than 1.0 EU/μg of bFGF as determined by the LAL method.

Biologic Activity

The ED50 is 0.1 to 1 ng/ml as determined by the dose dependent proliferation of NIH 3T3 cells.


Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.

Storage and Stability

Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at -20°C to -80°C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and up to 6 months when stored at -80°C.


Centrifuge briefly and then reconstitute bFGF in 500 μl of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/ml of bFGF. Avoid freeze-thaw cycles as it can result in loss of activity.

Related Products


  1. Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thompson, J.A. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227: 271-278.
  2. Xu, C., Rosler, E., Jiang, J., Lebkowski, J.S., Gold, J.D., O’Sullivan, C., Delavan-Boorsma, K., Mok, M., Bronstein, A., and Carpenter, M.K. (2005) Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23: 315-323.
  3. Vallier, L., Alexander, M., and Pedersen, R.A. (2005) Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J. Cell Sci. 118: 4495-4509.
  4. Wang, G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D., and Deng, H. (2005) Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330: 934-942.
  5. Greber, B., Lehrach, H., and Adjaye, J. (2007) Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal. Stem Cells 25: 455-464.

Additional Publications

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