Stemolecule™ ec23

Catalog Size Price Quantity
SRP002 5mg $67.83
SRP002-2 2 x 5mg $135.66
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ec23
ec23 Chemical Compound
ec23-induced Neurogenesis
Human TERA2.cl.SP12 EC cells exposed to either ec23 or ATRA form populations of terminally differentiated neurons after 21 days. Immunocytochemical staining for neurofilament-200 was performed on differentiated cultures.

Product Overview

Development of stable synthetic retinoid – ec23®

ReproCELL developed ec23 as a pan-RAR receptor agonist that is stable and maintains the same biological activity as ATRA (all trans retinoic acid). ATRA is well known for its ability to regulate cell differentiation. However, retinoids such as ATRA are excellent chromophores and efficiently absorb light and isomerise. This is not a desirable property for cell culture reagents where consistency and reliability of a molecule is important for robust, reproducible results. 

ReproCELL routinely test the stability of the our synthetic compounds in line with their development as stable alternatives to known biological molecules. The structural stability of  ec23 and ATRA were examined by NMR during prolonged exposure to light. ATRA shows significant isomerisation and degradation whereas ec23 remains completely intact.

Robust induction of neurogenesis by ec23

The synthetic retinoid, ec23 is a potent inducer of pluripotent stem cell differentiation. Human TERA2.cl.SP12 pluripotent stem cells exposed to either 10 μM ec23 or ATRA form populations of terminally differentiated neurons. Immunocytochemical staining for neurofilament-200 was performed on differentiated cultures and the number of positive cells quantified. Results show that ec23 is a more potent inducer of neurogenesis than ATRA. In direct comparison to ATRA,  synthetic retinoid ec23 induces larger numbers of neural cells with less variability and with the added advantage of compound stability.

Synthetic retinoid ec23 is also a potent inducer of neurogenesis by adult neuroprogenitor cells. Rat adult hippocampal progenitor cells (AHPCs) exposed to either 1 μM ec23 or ATRA differentiate into neural cell types. Differentiation induced by exposure to ec23 was consistent and reproducible.


Product Specifications

Size

5 mg (Cat. No. SR-P002)
10 mg (Cat. No. SR-P002-2)

Alternate Name

4-(5,5,8,8-Tetramethyl-5,6,7,8-tetrahydronaphthalen-2-ylethynyl) benzoic acid

Chemical Formula

C23H24O2

Molecular Weight

332 g/mol

CAS Number

104561-41-3

Purity

Greater than 99% by HPLC analysis

Formulation

Off-white solid

Solubility

Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.

Storage and Stability

Store powder at <20 °C. Following reconstitution, store aliquots at -20 °C. Stock solutions are stable for 6 months when stored as directed. 

Quality Control

The purity of ec23 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry.


Safety Data Sheets

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