Stemolecule™ IM-12

Catalog Size Price Quantity
04-0081 2 mg $84.00
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Im 12 Structure

Product Overview

The indolylmaleimide IM-12 is a potent enzymatic (IC50= 52 nM, compared with 92 nM for SB-216763) and intracellular inhibitor of GSK-3β which activates downstream components of canonical Wnt signaling and increasing β-catenin levels. IM-12 promotes increased differentiation of neural progenitor cells to neurons1. IM-12 has been shown to support the conversion and maintenance of human naïve stem cells2.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplment necessary to support naive pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor™ LIF, Human Recombinant (03-0016), and

A: Stemfactor™ Activin A, Human Recombinant (03-0001)


Product Specifications

Product Name

StemoleculeTM IM-12

Calalog Number

04-0081

Size

2 mg

Alternate Name

3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione

Chemical Formula

C22H20FN3O2

Molecular Weight

377.41

CAS Number

1129669-05-1

Purity

99% Purity by HPLC analysis

Appearance

Orange solid

Solubility

IM-12 is soluble in DMSO at 100 mM and in ethanol at 10 mM at room temperature.  For a 10 mM concentrated stock solution of IM-12, reconstitute the compound by adding 530 μL of DMSO to the entire contents of the vial.  If precipitate is observed, warm the solution to 37 C for 2 to 5 minutes. The maximum concentration recommended for dilution in media to ensure complete solubility is 10 μM For cell culture, the media should be pre-warmed prior to adding the reconstituted compountd. Note: for most cells, the maximum tolerance to DMSO is less than 0.5% by volume.

Storage and Stability

Store powder for up to six months at 4 C, protected from light. Following reconstitution, store aliquots at -20C.

Quality Control

The purity of IM-12 was determined by HPLC analysis.  The accurate mass was determined by mass spectrometry. Cellular toxicity of IM-12 was tested on mouse embryonic stem cells.


Specification Sheets

Safety Data Sheets



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