StemRNA™-NM Reprogramming Kit

Catalog Size Price Quantity
00-0076 1 kit $1,295.00
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ISSCR2016New_Fig6
P7 primary iPS cells derived from UPCs were stained using the StainAlive™ TRA-1-60 Antibody (09-0068) and DAPI after expansion in NutriStem™ XF/FF Culture Medium (01-0005)
ISSCR2016New_Fig5
The StemRNA-NM Reprogramming Kit
ISSCR2016New_Fig4
iPS cells derived from fibroblasts using the StemRNA-NM Reprogramming Kit were cultured on iMatrix-511 (NP892-011) in NutriStem™ XF/FF Culture Medium (01-0005) for 7 passages. Magnification: 4x

Product Overview

Third Generation RNA Reprogramming Technology for Cellular Reprogramming of Fibroblasts, Blood, and Urine

REPROCELL's latest evolution in the Stemgent RNA reprogramming technolgy combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
 

Key Benefits

  • Flexible technology generates high-quality human iPS cell lines from multiple target cell types
    Out-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived progenitor cells; UPCs)
  • High efficiency, non-integrating reprogramming
    StemRNA-NM requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, prividing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs).
  • Time-saving protocol delivers faster results facilitating higher throughput
    Colonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs.  No screening needed, saving an additional 2-10 weeks (3-10+ passages) needed for vector clearance with other technologies.
  StemRNA-NM (00-0076)
Feature Fibroblasts Urine (UPCs) Blood (EPCs)
No. wells per kit 9 3 3
No Transfections required 4 6-8 6-8
Days to primary iPS cell colonies 10-14 12-14 12-14
Reprogramming efficiency 2-+4% 0.1-0.5% 0.4-3%
Screening Required No No No
Xeno-compatible protocol Yes No No
GMP-compatible RNA manufacturing protocol Yes Yes Yes

StemRNA-NM_Timeline
Timeline for StemRNA-NM reprogramming of fibroblasts


Product Specifications

Size

1 kit

Kit Contents

  • OKSMNL NM-RNA (Part No. 05-0040), 30 µg, 1 vial
  • EKB NM-RNA (Part No. 05-0041), 22 µg, 1 vial
  • NM-microRNAs (Part No. 05-0042), 15 µg, 1 vial

Storage and Stability

  1. Store all three kit components at or below -70 °C.
  2. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.

Quality Control

The individual mRNAs are tested for size and integrity. The StemRNA™-SR Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts and human umbilical vein endothelial cells (HUVECs). Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for mycoplasma.

Limited Use Licenses

This product is intended for research use only. This product uses technology exclusively licensed from Biontech AG, as described in WO 2009/077134. This product may also be covered by one or more of WO 2007/036366, WO 2008/157688, WO 2012/072269, and WO 2014/072061, licensed from Biontech AG.

Limited Use License iPS Academia Japan

Recommended Usage

For use with associated StemRNA-NM protocols for generation of iPS cells.




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