Stemfactor™ Noggin, Human Recombinant

This product has been discontinued.

Product Overview

Noggin is a secreted homodimeric glycoprotein that binds to and inactivates members of the transforming growth factor-beta (TGF-β) superfamily of signaling proteins, such as Bone Morphogenetic Protein-4 (BMP-4)1. Noggin appears to have pleiotropic effects, both early in development as well as in later stages. The addition of Noggin during the culture of human embryonic stem (ES) cells in feeder-free medium supplemented with basic FGF inhibits BMP activity allowing human ES cells to maintain an undifferentiated, pluripotent state2-3.  Stemfactor Noggin is an approximate 65 kDa protein expressed in human cells as a glycosylated, disulfide-linked dimer. 


Product Specifications

Size

10 µg

Source

Stemfactor Noggin was expressed in, and purified from, human 293 cells.

Formulation

Lyophilized from sterile filtered 10 mM Tris, pH 7.4, 0.5 M NaCl.

Amino Acid Sequence

QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC 

Uniprot Accession Number

Q13253, residues 28-232

Purity

Greater than 95% by SDS-PAGE

Endotoxin Level

Less than 1.0 EU/μg of Noggin, as determined by the LAL method.

Biologic Activity

The ED50 is 20 to 80 ng/ml as determined by its ability to inhibit the BMP-4 (5.0 ng/ml) induced alkaline phosphatase production in ATDC-5 chondrogenic cells.

Sterility

Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.

Storage and Stability

Stemfactor Noggin is shipped at room temperature. Lyophilized Noggin is stable for up to 6 months from date of receipt when stored at -20°C to -80°C. Reconstituted Noggin, at concentrations greater than or equal to 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and up to 6 months when stored at -80°C.

Reconstitution

Centrifuge briefly and then reconstitute Nogginin water to yield a stock solution of 0.1 to 1.0 mg/ml. Avoid freeze-thaw cycles as it can result in loss of activity.


Specification Sheets

Safety Data Sheets

References

  1. Groppe, J., Greenwald, J., Wiater, E., Rodriguez-Leon, J., Economides, A.N., Kwiatkowski, W., Affolter, M., Vale, W.W., Belmonte, J.C., and Choe, S. (2002) Structural basis of BMP signaling inhibition by the cystine knot protein Noggin. Nature 420: 636-642.
  2. Xu, R.H., Peck, R.M., Li, D.S., Feng, X., Ludwig, T., and Thomson, J.A. (2005) Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2: 185-190.
  3. Wang, G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D., and Deng, H. (2005) Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330: 934-942.

Additional Publications



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