Stemfactor™ TGF-β1, Human Recombinant

Catalog Size Price Quantity
03-0004 5 μg $225.00
Add To Cart
Order Offline
T: 877.228.9783
F: 617.812.5900

Product Overview

Transforming Growth Factor beta 1 (TGF-β1) is a member of the TGF-β superfamily of multifunctional polypeptide growth factors that includes bone morphogenetic proteins (BMPs), Activin, and Nodal. TGF-β1 both helps maintain pluripotency in human embryonic stem (hES) cells and induces differentiation1,2. It also induces epithelial-mesenchymal transition and suppresses proliferation and differentiation of several types of lymphocytes, thus playing important roles in human cancers3. Stemfactor TGF-β1 is a 25 kDa homodimeric recombinant protein expressed in human HEK293 cells and composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.  

Product Specifications


5 μg


Stemfactor TGF-β1 was expressed in and purified from human HEK293 cells.


Lyophilized from sterile-filtered 50 mM NaOAc, pH 4.5, 200 mM NaCl.

Amino Acid Sequence


Uniprot Accession Number

P01137, residues 279-390.


Greater than 95% by SDS-PAGE analysis

Endotoxin Level

Less than 1.0 EU/μg of TGF-β1 as determined by the LAL method.

Biologic Activity

The ED50 is less than 0.2 ng/ml as determined by its ability to inhibit the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells.

Storage and Stability

Stemfactor TGF-β1 is shipped at room temperature. Lyophilized TGF-β1 is stable for up to 6 months from date of receipt when stored at -20°C to -80°C. Reconstituted TGF-β1, at concentrations greater than or equal to 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and up to 6 months when stored at -80°C.


Centrifuge briefly and then reconstitute TGF-β1 in 4 mM HCl to yield a stock solution of no less than 0.1 mg/ml. Avoid freeze-thaw cycles as it can result in loss of activity.

Specification Sheets

Safety Data Sheets

Stemgent Comments and Reviews (0)

No comments or reviews yet. Be the first to leave a review.

Add a Comment

You must log-in or register as a Stemgent member to submit a comment.

You are fully responsible for the content you post. We encourage collaboration and helpful reviews among scientists and will use this feature to conduct helpful reviews of our products. Content that includes profanity, personal attacks or antisocial behavior (such as "spamming" or "trolling"), or other inappropriate content or material will be removed. We reserve the right to block any user who violates our terms of use, including removing all content posted by that user.