Stemfactor™ BMP-4, Human Recombinant

Catalog Size Price Quantity
03-0007 10 μg $219.00
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Product Overview

Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGF-β protein super-family. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair1. In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishment of a dorsal-ventral axis2,3. BMP-4 plays an important role indifferentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal4. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human ES cells5.  Stemfactor BMP-4 is a recombinant protein expressed and purified from human 293 cells as a glycosylated homodimer with a molecular mass of 34 kDa. 


Product Specifications

Size

10 µg

Source

Stemfactor BMP-4 was expressed in and purified from human 293 cells.

Formulation

Lyophilized from sterile-filtered 50 mM NaOAc, pH 4.5 and 1 M NaCl.

Amino Acid Sequence

SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR

Uniprot Accession Number

P12644, residues 293-408

Purity

Greater than 95% by SDS-PAGE analysis.

Endotoxin Level

Less than 1.0 EU/μg of BMP-4 as determined by the LAL method.

Biologic Activity

The ED50 is less than 30 ng/ml as determined by its ability to induce alkaline phosphatase production by mouse chondrogenic ATDC-5 cells.

Sterility

Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.

Storage and Stability

Stemfactor BMP-4 is shipped at room temperature. Lyophilized BMP-4 is stable for up to 6 months from date of receipt when stored at -20°C to -80°C. Reconstituted BMP-4, at concentrations greater than or equal to 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and up to 6 months when stored at -80°C.

Reconstitution

Centrifuge briefly and then reconstitute BMP-4 in 4 mM HCl to yield a stock solution of no less than 0.1 mg/ml. Avoid freeze-thaw cycles as it can result in loss of activity.


Specification Sheets

Safety Data Sheets

References

  1. Miljkovic, N.D., Cooper, G.M., and Marra, K.G. (2008) Chondrogenesis, bone morphogenetic protein-4 and mesenchymal stem cells. Osteoarthritis Cartilage 16: 1121-1130.
  2. Chen, D., Zhao, M., and Mundy, G.R. (2004) Bone morphogenetic proteins. Growth Factors 22: 233-241.
  3. Sadlon, T.J., Lewis, I.D., and D’Andrea, R.J. (2004) BMP4: its role in development of the hematopoietic system and potential as a hematopoietic growth factor. Stem Cells 22: 457-474.
  4. Ying, Q.L., Nichols, J., Chambers, I., and Smith, A. (2003) BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115: 281-292.
  5. Xu, R.H., Chen, X., Li, D.S., Li, R., Addicks, G.C., Glennon, C., Zwaka, T.P., and Thompson, J.A. (2002) BMP4 initiates human embryonic stem cell differentiation to trophoblasts. Nat Biotechnol. 20: 1261-1264.

Additional Publications



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