Stemolecule™ BIO

Catalog Size Price Quantity
04-0003 2 mg $99.00
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Product Overview

BIO (6-bromoindirubin-3'-oxime) is a potent, reversible and ATP-competitive inhibitor for GSK-3α/β in the Wnt signaling pathway. BIO is the first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells1. BIO-mediated activation of the Wnt signaling cascade maintained the undifferentiated state of both mouse and human embryonic stem cells under feeder free conditions possibly by Wnt-induced increase in the expression of Nodal2-4 


Product Specifications

Size

2 mg

Alternate Name

(2'Z,3'E)-5-Bromoindirubin-3'-oxime 

Chemical Formula

C16H10BrN3O2

Molecular Weight

356.17

CAS Number

667463-62-9

Purity

Greater than 98% by HPLC analysis 

Formulation

Dark red solid

Solubility

For a 10 mM concentrated stock solution of BIO, reconstitute the compound by adding 561.5 μl of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM and ethanol at 10 mM.   

Storage and Stability

Store powder at either 4°C or -20°C, protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality Control

The purity of BIO was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of BIO was tested on mouse embryonic stem cells. 


Specification Sheets

Safety Data Sheets



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