NutriStem™ XF/FF Culture Medium for Human iPS and ES Cells

Catalog Size Price Quantity
01-0005 500 ml $230.00
01-0005-100 100 ml $67.00
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NutriStem
Morphology of Human H1 ES cells Grown in NutriStem XF/FF for 20 passages.
01 0005 Figure 2
Expression of Pluripotency Marker in hES Cells Culured in NutriStem XF/FF.
01 0005 Figure 3
Proliferation Growth rate of human H1 ES cells cultured in NutriStem XF/FF Culture Medium versus competitor mTeSR1™.

Product Overview

Notice to clinical users:  A Drug Master File for NutriStem medium has been filed with the US FDA.

Please contact us for more information. (info@reprocellusa.com)

 

NutriStem XF/FF Culture Medium is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo. NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications.

  • Xeno-free, feeder-free conditions contain no animal components
  • Low basic FGF (4 ng/ml), and TGFβ (<5 ng/ml)
  • Easy, one-step transition from feeder-dependent culture, no adaptation required
  • Maintains pluripotency, normal morphology, karyotype, and differentiation potential of human ES and iPS cells over long term culture
  • Robust attachment and high cloning efficiency from single cells

Product Specifications

Size

100 ml (Cat. No. 01-0005-100)
500 ml (Cat. No. 01-0005)

Storage and Stability

Store at -20°C. This product is stable for a minimum of 3 months when stored as directed.

Quality Control

NutriStem Medium tested negative for mycoplasma. Human ES cells expressed the pluripotency markers SSEA-4, TRA-1-60, and TRA-1-81 after three continuous passages in NutriStem Medium.

Trademarks

NutriStem is a trademark of Biological Industries.


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Stemgent Comments and Reviews (1)

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jim chen good products
Jan 1, 2014

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