Stemolecule™ BIX01294

Catalog Size Price Quantity
04-0002 5 mg $104.00
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04-0002 Figure 1

Product Overview

BIX01294 is a histone lysine methyltransferase (HMTase) inhibitor that selectively inhibits the G9a HMTase and the generation of histone H3 lysine 9 methylation (H3K9me2)1. BIX01294 has also been found to play a role in the production of induced pluripotent stem (iPS) cells. The reprogramming efficiency of nerual progenitor cells (NPCs) transduced with Oct4 and Klf4 was found to be significantly improved by the addition of BIX01294, suggesting that it can replace one or more viral factors2. This small molecule can be a useful tool in the study of hystone lysine methylation, reprogramming, and differentiation. 


Product Specifications

Size

5 mg

Alternate Name

2-(Hexahydro-4-methyl-1H-1,4-diazepin-1-yl)-6,7-dimethoxy-N-[1-(phenylmethyl)-4-piperidinyl]4-quinazolinamine

Chemical Formula

C28H38N6O2 

Molecular Weight

490.64 

CAS Number

935693-62-2 

Purity

Greater than 94% by HPLC analysis 

Formulation

Pale yellow solid 

Solubility

For a 10 mM concentrated stock solution of BIX01294, reconstitute the compound by adding 1.02 ml of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.   

Storage and Stability

Store powder at either 4°C or -20°C, protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. 

Quality Control

The purity of BIX01294 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry.  Cellular toxicity of BIX01294 was tested on mouse embryonic stem cells. 


Specification Sheets

Safety Data Sheets

References

  1. Kubicek, S., O’Sullivan, R.J., August, E.M., Hickey, E.R., Zhang, Q., Teodoro, M.C., Rea, S., Mechtler, K., Kowaslki, J.A., Homon, C.A., Kelly, T.A., and Jenuwein, T. (2007) Reversal of H3K9me2 by a small-molecule inhibitor for the G9a histone methyltransferase. Mol Cell 25: 473-481.
  2. Shi, Y., Do, J.T., Desponts, C., Hahm, H.S., Schöler, H.R., and Ding, S. (2008) A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2: 525-528.
  3. Shi, Y., Desponts, C., Do, J.T., Hahm, H.S., Schöler, H.R., and Ding, S. (2008) Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3: 568-574.

Additional Publications



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